Uniparental disomy in ES cells
Homozygous mutant cells can be generated from embryonic stem (ES) cells with a single insertion of a drug-resistance marker by increasing the concentration of the selection drug. In the March Nature Genetics, Lefebvre et al. report analysis of the mechanism governing this loss of heterozygosity (LOH) (Nature Genetics 2001, 27:257-258). They used an ES cell line resulting from a cross between two different inbred mouse 129 substrains which could be distinguished by single sequence-length polymorp
Mar 6, 2001
Homozygous mutant cells can be generated from embryonic stem (ES) cells with a single insertion of a drug-resistance marker by increasing the concentration of the selection drug. In the March