Wiring up
In March 8 Nature Philip Leighton and colleagues describe a large-scale screen for molecules that guide axons during the development of the nervous system in mice (Nature 2001, 410:174-179). They developed a gene-trap screening method that incorporates elements of the 'secretory trap' technique combined with an axonal marker (placental alkaline phosphatase) whose translation is driven by an IRES (internal ribosome entry site).The method enabled the generation of a large number of mouse lines wit
Mar 8, 2001
In March 8
The method enabled the generation of a large number of mouse lines with diverse patterns of axon labelling in the brain. By comparing the axon tract staining patterns in heterozygous and homozygous mutant mice, Leighton