Department of Microbiology & Immunology
University of Illinois

  • Cosy, Noesy, and HoHaHa! Respectively, these are abbreviations for two-dimensional scalar correlated spectroscopy, two-dimensional nuclear Overhauser enhancement spectroscopy, and Homonuclear Hartmann Hahn transfer experiment - three of the techniques used to study Megasphaera elsdenii. Apparently, we need to learn well the general rules for two-dimensional nuclear magnetic resonance (NMR) determination of atomic connectivities and three-dimensional polypeptide structures. Assignment of the 1H NMR resonances of the 137 amino acid residues of flavodoxin is a good lesson as well as a careful example of the power of two-dimensional NMR structural determinations for noncrystalline proteins.

    C.P.M. van Mierlo, J. Vervoort, F. Müller, A. Bacher, "A two-dimensional 1H NMR study on Megasphaera elsdenii flavodoxin in the reduced state," European Journal of Biochemistry, 187, 521-41, February 1990. (Agricultural University, Wageningen, The Netherlands; Technical University of Munich)

  • Many scientists are modeling membrane protein folding either...

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