Life Sciences

LIFE SCIENCES BY SIMON SILVER Department of Microbiology & Immunology University of Illinois Chicago, Ill. The protein secretory apparatus E. coli is being taken apart and put together in vitro like a Tinkertoy: SecA is a peripheral membrant ATPase of 102 kdaltons. SecB is separate and exists as a cytoplasmic tetramer of 16x4=64 kdaltons that is sufficient by itself for transport of some exported polypeptides. M. Watanabe, G. Blobel, “Cytosolic factor purified from Escherichia coli is

Simon Silver
Jun 11, 1989

LIFE SCIENCES

BY SIMON SILVER
Department of Microbiology & Immunology
University of Illinois
Chicago, Ill.

The protein secretory apparatus E. coli is being taken apart and put together in vitro like a Tinkertoy: SecA is a peripheral membrant ATPase of 102 kdaltons. SecB is separate and exists as a cytoplasmic tetramer of 16x4=64 kdaltons that is sufficient by itself for transport of some exported polypeptides.

M. Watanabe, G. Blobel, “Cytosolic factor purified from Escherichia coli is necessary and sufficient for the export of a preprotein and is a homotetramer of SecB,” Proceedings of the National Academy of Sciences, USA, 86,2728-32, April 1989. (The Rockefeller University, New York, N.Y.)

K. Cunningham, R. Lill, E. Crooke, M. Rice, et al., “SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA,” EMBO Journal, 8. 955-9, March 1989. (University...

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