Notable

M. Gray, S.M. Honigberg, "Effect of chromosomal locus, GC content and length of homology on PCR-mediated targeted gene replacement in Saccharomyces," Nucleic Acids Research, 29:5156-62, Dec. 15, 2001. F1000 Rating: Recommended "While gene knockouts or replacements are easily and commonly done in yeast, the targeting efficiency is quite variable. The authors have systematically evaluated key parameters and found that a GC content of at least 40% in the regions of homology improves targeting ef

Jeffrey Perkel
Mar 17, 2002
M. Gray, S.M. Honigberg, "Effect of chromosomal locus, GC content and length of homology on PCR-mediated targeted gene replacement in Saccharomyces," Nucleic Acids Research, 29:5156-62, Dec. 15, 2001.

F1000 Rating: Recommended

"While gene knockouts or replacements are easily and commonly done in yeast, the targeting efficiency is quite variable. The authors have systematically evaluated key parameters and found that a GC content of at least 40% in the regions of homology improves targeting efficiency. Surprisingly, changing the length of targeting homology from 40 to 60 bp had little effect."

—Janet Lindsley,
University of Utah, US

Genomes & Genomics

R. Tompa et al., "Genome-wide profiling of DNA methylation reveals transposon targets of CHROMOMETHYLASE3," Current Biology,12:65-8, Jan. 8, 2002.

F1000 Rating: Recommended

"A new technique can be used to monitor cytosine methylation across many genomic sites using microarrays. Using this technique, the authors investigate the targets...

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