Menu

Youssef Belkhadir Deciphers Plants’ Signaling Soundtrack

An entrepreneurial attitude helped this Vienna-based researcher begin to unravel the complex receptor network that Arabidopsis uses to develop and defend itself.

Jun 1, 2018
Ashley Yeager

© HANS HOCHSTÖGERGrowing up in Morocco, Youssef Belkhadir would look out across the wheat fields of his father’s farm outside Casablanca in wonder. “I was fascinated by how plants managed to colonize the environment in such an elegant way, making the most of everything,” Belkhadir tells The Scientist. “They are tethered in the ground and cannot escape their environment through locomotion. Their site of birth will be their site of death, and they have to deal with whatever comes their way.”

Inspired by this idea, Belkhadir decided to study the molecular biology of plants as an undergraduate at the University of Paris VI-Jussieu. After receiving his bachelor’s in 2001, he spent another year at the University of Paris-Sud XI earning his master’s degree in plant genomics, before moving to the U.S. to join biologist Jeffery Dangl’s lab at the University of North Carolina at Chapel Hill for his PhD. There, Belkhadir investigated how plants sense and defend themselves against pathogen attack using molecular machines inside each cell.1 “Even then, Youssef was very big-thinking and tenacious,” Dangl recalls.

After earning his PhD in 2005, Belkhadir joined the lab of biologist Joanne Chory at the Salk Institute for Biological Studies in La Jolla, California, as a research associate, to study how plants respond to steroid signals. “What Youssef learned in my lab was how to take a lot of knocks, and still move forward,” Chory says. “At first, all of his projects failed, not because they were bad projects, but they just didn’t work. Youssef just seemed to laugh through it all.” Finally, success came while studying a leucine-rich repeat receptor kinase (LRR-RK), which acts as an antenna at the cell surface to bind to a steroid hormone that helps plants control their size.2 “I got my affinity for cell surface receptors in Dr. Chory’s lab,” Belkhadir says. “But I didn’t just want to study one at a time. I wanted to study hundreds at a time.”

At the end of 2010, Belkhadir planned to tackle this problem as an independent researcher at the Centre of Excellence in Plant Energy Biology in Perth. But he never made it to Australia. While he was in California, tragedy stuck: Belkhadir found out that his father had been in a traffic accident, so he returned to Morocco to be with his family. After his father passed away, Belkhadir remained in his native country to settle his father’s affairs, which kept him away from the lab bench for more than three years.

To stay engaged in science, Belkhadir cofounded a biotech company called Atlas Genomics with his own money—a venture he says taught him to be excruciatingly efficient and to outsource tasks when necessary. After a few years, however, Belkhadir chose to shut down the firm for financial reasons, partly related to the Arab Spring demonstrations that raged through 2011. It was time to return to the lab. “It’s incredibly hard to come back after years away,” Chory says. “But Jeffrey Dangl and I went to bat for Youssef because we knew he could do it.”

When Belkhadir resumed his research in 2014, he accepted a position as a group leader at the Gregor Mendel Institute in Austria to study how plants use cell surface receptors to adapt to their environment. Just a few years later, he had successfully completed several projects: three identified the peptide ligands of three independent classes of receptors that regulate the output of plant immune responses, and another identified a receptor that ensures healthy root development. Simultaneously, Belkhadir and his colleagues developed the tools to trace 40,000 interactions among 200 LRR-RKs and discovered a complex network of surface receptors that distinguishes friendly extracellular molecules from pathogenic ones.3

Now, Belkhadir says, the question is: When multiple signals activate the network, how do plants compute the signals to make the best decisions to defend themselves or grow?

References

  1. B.F. Holt III et al., “Antagonistic control of disease resistance protein stability in the plant immune system,” Science, 309:929-32, 2005. (Cited 221 times)
  2. Y. Belkhadir, J. Chory, “Brassinosteroid signaling: A paradigm for steroid hormone signaling from the cell surface,” Science, 314:1410-11, 2006. (Cited 147 times)
  3. E. Smakowska-Luzan et al., “An extracellular network of Arabidopsis leucine-rich repeat receptor kinases,” Nature, 553:342-46, 2018. (Cited 5 times)

January 2019

Cannabis on Board

Research suggests ill effects of cannabinoids in the womb

Marketplace

Sponsored Product Updates

FORMULATRIX® digital PCR technology to be acquired by QIAGEN
FORMULATRIX® digital PCR technology to be acquired by QIAGEN
FORMULATRIX has announced that their digital PCR assets, including the CONSTELLATION® series of instruments, is being acquired by QIAGEN N.V. (NYSE: QGEN, Frankfurt Stock Exchange: QIA) for up to $260 million ($125 million upfront payment and $135 million of milestones).  QIAGEN has announced plans for a global launch in 2020 of a new series of digital PCR platforms that utilize the advanced dPCR technology developed by FORMULATRIX combined with QIAGEN’s expertise in assay development and automation.
Application of CRISPR/Cas to the Generation of Genetically Engineered Mice
Application of CRISPR/Cas to the Generation of Genetically Engineered Mice
With this application note from Taconic, learn about the power that the CRISPR/Cas system has to revolutionize the field of custom mouse model generation!
Translational Models of Obesity, Dysmetabolism, Diabetes, and Complications
Translational Models of Obesity, Dysmetabolism, Diabetes, and Complications
This webinar, from Crown Bioscience, presents a unique continuum of translational dysmetabolic platforms that more closely mimic human disease. Learn about using next-generation rodent and spontaneously diabetic non-human primate models to accurately model human-relevant disease progression and complications related to obesity and diabetes here!
BiochemAR: an augmented reality app for easy visualization of virtual 3D molecular models
BiochemAR: an augmented reality app for easy visualization of virtual 3D molecular models
Have you played Pokemon Go? Then you've used Augmented Reality (AR) technology! AR technology holds substantial promise and potential for providing a low-cost, easy to use digital platform for the manipulation of virtual 3D objects, including 3D models of biological macromolecules.