Incomplete microbial genome assemblies can limit what researchers are able to interpret from sequencing data. When complex genomic regions remain unresolved, the context needed to determine pathogen identity, detect resistance and virulence factors, and investigate transmission pathways may be missed.
Long-read sequencing can enable complete genomic assemblies and improve characterization across a range of applications. This guide connects these scientific benefits to practical workflow decisions, helping researchers evaluate sequencing approaches based on sample type, experimental goals, and downstream analysis requirements.
Download this guide to explore
- How long-read technology supports complete microbial genome and plasmid sequencing
- Workflow options for isolates, amplicons, colonies, and viruses
- Library prep considerations for mixed microbial communities
- Analysis workflows for metagenomic and single microbial genomes
