Western blotting is one of the most well-known and commonly used techniques in bioscience laboratories today. However, the Western blotting workflow is quite nuanced, involves multiple components, and can be tricky to optimize. Furthermore, despite being around for decades, Western blotting continues to evolve, presenting the researcher with more choices and options than ever.
Download this eBook from The Scientist to learn about:
• How to prepare samples for Western blotting
• The anatomy of a protein electrophoresis gel
• Picking the right membrane for your protein transfer
• Tips to selecting the best antibody for optimizing signal detection
This free eBook was made possible by an educational grant from MilliporeSigma.