Protein Prep 101 - Extracting Value

Learn about common challenges to protein preparation protocols, typical protein isolation workflows, considerations when working with challenging samples, and how to confirm intact protein extraction with this eBook!

May 9, 2018
The Scientist Creative Services Team

If DNA is the blueprint of life, then proteins comprise the edifices themselves. Naturally, the study of proteins – their structures, abundance, function, and interactions – has proven integral to our understanding of biology. Protein-centered research is pivotal to human health and disease, and protein-based products are consumed commercially, used in academic research, and serve as biopharmaceuticals.

To effectively study proteins or manufacture protein-based products, researchers need to be able to detect, characterize, and quantify the protein(s) of interest. The actual steps involved in protein extraction and preparation protocols will depend on a host of factors, but all protein preparation protocols strive to optimize two main parameters: yield and purity.

Download this eBook from The Scientist and MilliporeSigma to learn about:
 • Common challenges to protein preparation protocols
 • A typical protein isolation workflow
 • Considerations when working with challenging samples
 • Confirming intact protein extraction