Menu

Real-Time Protein Dynamics with Live-Cell Immunocytochemistry

Learn about the importance and evolution of immunocytochemistry, adapting ICC for live-cell analysis, using ICC to expand your protein analysis toolkit, and the value of ICC for disease research!

May 25, 2018
The Scientist Creative Services Team

Thanks to the power and utility of immunocytochemistry, we are now able to obtain information about protein presence, location, and activity - information instrumental to our capacity to identify cellular phenotypes and understand the mechanisms that govern cell behavior.

Live-cell analysis has been used extensively to study cellular behavior, but has not been a popular option for studying protein expression dynamics. This is now changing, as technological advancements are facilitating modified ICC protocols that embrace live-cell analysis, adding a much valued temporal dimension to ICC-obtained data.

Live-cell ICC permits repeated sampling of the same cell(s) over time, building upon traditional ICC by expanding on the 'before and after' dichotomy. It gives researchers the ability to identify the precise stimuli involved in modifying protein expression levels and the temporal kinetics of these changes.

Download this original eBook from The Scientist to learn more about:
 • The importance and evolution of immunocytochemistry
 • Adapting ICC for live-cell analysis
 • Using ICC to expand your protein analysis toolkit
 • ICC for disease research

This free eBook was made possible by an educational grant from IncuCyte, a Sartorius Brand.

September 2018

The Muscle Issue

The dynamic tissue reveals its secrets

Marketplace

Sponsored Product Updates

Horizon Discovery introduces Myeloid DNA Reference Standard to support genetic testing of leukemia

Horizon Discovery introduces Myeloid DNA Reference Standard to support genetic testing of leukemia

Horizon Discovery Group plc, a global leader in gene editing and gene modulation technologies, today announced the launch of its Myeloid DNA Reference Standard. The first-to-market large cell-line derived myeloid cancer reference standard designed enables faster, more reliable and more cost-effective assay validation, to support the market in bringing routine testing into practice.

StemExpress LeukopakâNow Available in Frozen Format

StemExpress LeukopakâNow Available in Frozen Format

StemExpress, a Folsom, California based leading supplier of human biospecimens, announces the release of frozen Peripheral Blood Leukopaks. Leukopaks provide an enriched source of peripheral blood mononuclear cells (PBMCs) with low granulocyte and red blood cells that can be used in a variety of downstream cell-based applications.

New Antifade Mounting Media from Vector Laboratories Enhances Immunofluorescence Applications

New Antifade Mounting Media from Vector Laboratories Enhances Immunofluorescence Applications

Vector Laboratories, a leader in the development and manufacture of labeling and detection reagents for biomedical research, introduces VECTASHIELD® Vibrance™ – antifade mounting media that delivers significant improvements to the immunofluorescence workflow.

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Download this white paper from Bertin Technologies to learn how to extract and analyze lipid samples from various models!