Single-cell CRISPR screens enable the exploration of mammalian gene function and genetic regulatory networks. Recently, multiple techniques have emerged that pair CRISPR screens with high-throughput single-cell RNA sequencing (scRNA-seq), resulting in high resolution, information-rich readouts.
In this webinar brought to you by The Scientist and sponsored by 10x Genomics, Dina Finan will introduce the 10x Genomics product portfolio, including new targeted gene expression panels. Joining Dina will be Joseph Replogle who will discuss how this technology helped him and colleagues develop direct capture Perturb-seq, a new single cell CRISPR screening technique that greatly expands accessibility, scalability, and flexibility of single cell CRISPR experiments.
Topics to be covered
- Streamlining pooled single-cell CRISPR screens with combinatorial perturbation libraries
- Improving efficacy of CRISPR interference and activation by targeting individual genes with multiple sgRNAs
- Applying direct capture Perturb-seq to study the genetic interactions (GIs) between cholesterol biosynthesis and DNA repair genes
- Leveraging hybridization-based target enrichment gene panels to decrease sequencing cost up to 90%
Meet the Speakers:
Joseph Replogle, MD/PhD trainee
Dina Finan, PhD