Biomolecule purification involves a complex series of steps where target purity and recovery are vital. Many common nucleic acid and protein handling procedures can benefit from the use of centrifugal devices outfitted with microfiltration or ultrafiltration membranes. However, finding the right products can be challenging. This webinar, sponsored by Pall Laboratory, will highlight ways to simplify purification in the molecular biology lab.
Topics to be Covered
- The interplay between membrane selection, sample composition, and centrifugation conditions
- Device choices available for processing nucleic acid and protein samples ranging in volume from 50 µL to 60 mL
- Nucleic acid applications
- Cleanup of DNA fragments, such as PCR products, prior to restriction digestion and labeling reactions
- Gel purification of DNA fragments
- Protein applications
- Concentration, fractionation, and desalting or buffer exchange (diafiltration)
- Elution of proteins from polyacrylamide gels
Meet the Speaker:
Jorgen De Haan, PhD
Senior Application Scientist
Jorgen de Haan received his Ph.D. in Biochemistry from the University of Illinois at Urbana-Champaign, where he studied the Molecular mechanisms of Estrogen Receptor action, and performed postdoctoral work at the Wyeth-Ayerst Research Women’s Health Research Institute and at the University of Michigan. As a research Scientist, Jorgen worked at the Broad Institute and at Massachusetts General Hospital. Jorgen de Haan currently is a Senior Applications Scientist in Pall’s Scientific Laboratory Services group.