Hematopoietic stem and progenitor cells (HSPCs) are capable of generating a large diversity of blood cells throughout the human lifespan. Although these cells have been extensively evaluated, there remains significant uncertainty in the degree of heterogeneity within HSPC subpopulations and their associated differentiation trajectories. Identifying HSPC phenotypic diversity is important for better understanding the pathogenesis of numerous blood disorders, including hematologic malignancies.
In this webinar brought to you by The Scientist and BD Biosciences, Margaret Nakamoto will introduce the BD Rhapsody™ Single-Cell Analysis System with whole transcriptome analysis (WTA) and BD® AbSeq Antibody-oligo surface marker evaluation, including the data workflow ranging from sample preparation to cell type identification and expression analyses. Ravi Majeti will then discuss how he and his team used concurrent RNA and BD® AbSeq Antibody-oligo multiplexed surface marker analysis to improve HSPC clustering and characterize specific phenotypic states along unique hematopoietic differentiation trajectories.
Topics to be covered
- Application of single-cell multiomics to healthy bone marrow mononuclear cells (BMMCs)
- Identification of cell type clusters and surface marker associations using combined single-cell whole transcriptome amplification and surface marker analysis
- Correlation of cell types with both canonical and novel surface markers and how deconvolution analysis provides preliminary insights into their potential clinical relevance in acute myeloid leukemia (AML)
Meet the Speakers:
Margaret Nakamoto, PhD
Ravi Majeti, MD, PhD
Professor of Medicine
Chief, Division of Hematology
Institute for Stem Cell Biology and Regenerative Medicine
Stanford University School of Medicine