PCR is a beneficial laboratory tool; however, its efficiency is only as robust as the reaction parts, including the nucleic acid template. The most common methods for nucleic acid isolation use silica spin columns or magnetic silica beads. These methods are convenient because of commercially available kits, and researchers perform them safely without a fume hood. However, they involve tedious binding and washing steps, error-prone tube handling, and cross contamination risks.
What if those bind and wash steps could be eliminated from the workflow? This webinar, sponsored by MilliporeSigma, will demonstrate the benefits of a new negative chromatography method of nucleic acid isolation using GenElute™-E single spin technology. Focusing on the extraction and isolation of high purity, high quality nucleic acid template with minimal chemical and biological contaminants helps prevent inaccurate sample quantitation and unusual downstream results.
Topics to be covered
- How chemical and biological impurities introduced during isolation and preparation of the nucleic acid template negatively affect DNA or RNA quantitation and PCR
- The principle of negative chromatography via size exclusion for DNA and RNA purification
- Commonly overlooked factors that impact PCR robustness and qPCR accuracy
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