A cause for concern regarding the popular CRISPR-Cas9 technology is the potential occurrence of off-target effects. RNA-guided Cas9 may cleave DNA sequences that are not exact complements of the guide strand when either strand harbors bulges due to insertions or deletions. Undoubtedly, a better understanding of the specificity of the CRISPR-Cas9 system can help develop strategies to minimize off-target cleavage. To take a closer look at the specificity and possible causes of off-target effects in the CRISPR-Cas9 system, The Scientist is bringing together researchers who will summarize their work on off-target effects limiting the applications of Cas9-mediated genome modification and discuss strategies for predicting and preventing such off-target effects. Attendees will have the unique opportunity of asking experts about their experience with off-target effects of the CRISPR-Cas9 system.
Topics to be covered:
- Whole genome sequencing to assess Cas9 off-target effects in genetically engineered mice
- Will elimination of off-target effects be good enough?
Meet the Speakers:
Lauryl Nutter, PhD
Associate Director, Model Production and Cryopreservation & Recovery
The Centre for Phenogenomics
The Hospital for Sick Children