Menu

Phenotypic CRISPR Screening: Looking Beyond Cell Viability To Study Complex Genetic Interactions

Horizon Discovery invites you to join them for an educational webinar.

Sep 6, 2018
The Scientist Creative Services Team

FREE Webinar

Tuesday, October 30, 2018
2:30-4:00 PM Eastern Time
Register Now

CRISPR screening, whether using knockout, activation, or inhibition approaches, has become an essential tool for drug discovery. A powerful adaptation of CRISPR screening is created by coupling pooled NGS-linked or arrayed screening strategies with phenotypic measurements. By using an advanced combination of techniques, this screening strategy can be targeted to detect genes that drive complex biological responses via biomarker signal readout or a panel of high content parameters. Scientists at Horizon, the sponsor of this LabTools webinar, have developed both FACS-linked and HCA-based strategies for analysis where cellular pathophysiology is uncoupled from cell health, providing much richer and biologically relevant outcomes. In this webinar, we will discuss data obtained and strategies learned from several successful screens and present ongoing work using increasingly relevant cell models, such as T cells and using single cell RNAseq-based screening approaches.

Topics to be covered:

  • Discussion of the strengths and considerations associated with complex phenotypic screens
  • Examples of successful applications of pooled and arrayed phenotypic screens

Register Now

Meet the Speakers:

Benedict Cross, PhD
Head of Functional Genomics
Horizon Discovery



Carlos le Sage, PhD
Team Leader
Horizon Discovery



Steffen Lawo, PhD
Team Leader
Horizon Discovery



October 2018

Bright Lights, Big Problems

Scientists are exploring the ecological damage caused by artificially lit night skies

Marketplace

Sponsored Product Updates

Neural Cell Culturing Guide

Neural Cell Culturing Guide

Download this guide from R&D Systems to learn the fundamental techniques and protocols for successfully isolating, culturing, and expanding specific cell types from the rodent central and peripheral nervous systems!

Immunophenotyping Extracellular Vesicles Using Amnis® Technology

Immunophenotyping Extracellular Vesicles Using Amnis® Technology

Download this application note to learn how the Amnis® time delay integration (TDI) image capturing system facilitates high-throughput flow cytometry with high sensitivity to submicron particles!

Improving Accuracy in Serial Dilutions Using the Electronic Pipette

Improving Accuracy in Serial Dilutions Using the Electronic Pipette

Electronic pipettes provide more accurate sample concentrations, reduced error propagation, and improved ergonomics. Download this application note from Sartorius to learn more!

Benefits of Electronic Pipette Use for Pipetting PCR Master Mix

Benefits of Electronic Pipette Use for Pipetting PCR Master Mix

Download this application note from Sartorius to explore how electronic pipettes can help ensure reproducible and reliable results when performing PCR-based assays.