Menu

Power Up! CRISPRi & CRISPRa Tools for Genome-Wide Screening

Horizon Discovery is leveraging these technologies to address novel research questions and deliver insights into essential gene function, hypomorphic expression, and gene dominance.

Aug 2, 2017
The Scientist Creative Services Team

Power Up! CRISPRi & CRISPRa Tools for Genome-Wide Screening from thescientistllc on Vimeo.

Forward genetic screening with CRISPR-Cas9 has created remarkable new opportunities for biological discovery. The power of complete gene knockout in a pooled screening platform has delivered novel target ID, tackled complex mechanism of action, and driven the design of efficient and economical patient stratification for clinical studies. Using transcriptional regulation with catalytically-dead Cas9 (dCas9), both loss-of-function studies (CRISPR interference, CRISPRi) and gain-of-function studies (CRISPR activation, or CRISPRa) are now possible at a genome-wide level. Horizon Discovery is leveraging these technologies to address novel research questions and deliver insights into essential gene function, hypomorphic expression, and gene dominance. In this webinar we will introduce these new powerful tools and share our screening datasets. Attendees can interact with our expert speaker during the live webinar, asking questions and discussing their experiences.

Topics to be covered:

  • Introduction to CRISPRi and CRISPRa and their use in functional genomic screening
  • How to use CRISPRi/a screening for target ID and validation, understanding drug MOA and patient stratification
  • Screen design and how to evaluate success of your screening program
  • Example screening datasets and how to analyse and interpret the data

View the Video Now

Meet the Speaker:

Benedict Cross, PhD
Functional Genomic Screening Lead
Horizon Discovery

 

 

 

 

 

 

 

April 2019

Will Car T Cells Smash Tumors?

New trials take the therapy beyond the blood

Marketplace

Sponsored Product Updates

Myth Busting: The Best Way to Use Pure Water in the Lab
Myth Busting: The Best Way to Use Pure Water in the Lab
Download this white paper from ELGA LabWater to learn about the role of pure water in the laboratory and the advantages of in-house water purification!
Shimadzu's New Nexera UHPLC Series with AI and IoT Enhancements Sets Industry Standard for Intelligence, Efficiency and Design
Shimadzu's New Nexera UHPLC Series with AI and IoT Enhancements Sets Industry Standard for Intelligence, Efficiency and Design
Shimadzu Corporation announces the release of the Nexera Ultra High-Performance Liquid Chromatograph series, incorporating artificial intelligence as Analytical Intelligence, allowing systems to detect and resolve issues automatically. The Nexera series makes lab management simple by integrating IoT and device networking, enabling users to easily review instrument status, optimize resource allocation, and achieve higher throughput.
IDT lowers genomic barriers with powerful rhAmpSeq™ targeted sequencing system
IDT lowers genomic barriers with powerful rhAmpSeq™ targeted sequencing system
Increasing accuracy and reducing cost barriers, IDT’s innovative system delivers simple and cost-effective amplicon sequencing
Bio-Rad Introduces Isotype-Specific Secondary Antibodies
Bio-Rad Introduces Isotype-Specific Secondary Antibodies
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb), a global leader of life science research and clinical diagnostic products, today announced the launch of its isotype-specific secondary antibodies. This new range of recombinant monoclonal antibodies, directed against the three main mouse isotypes: IgG1, IgG2a, and IgG2b, offer improved signal detection and specificity in imaging, ELISA, flow cytometry, and western blotting.