G-protein coupled receptors (GPCRs), a class of membrane proteins, are involved in critical cellular signaling pathways that are linked to the onset and cause of many diseases. Determining the structure and function of membrane proteins can be challenging, partly due to their hydrophobic properties, which make solubilization difficult. QTY variants have 46-56 percent of their transmembrane α-helices altered, but they are water-soluble, maintain stable structures, are biologically functional, and retain proper ligand-binding activity.
In this webinar sponsored by NanoTemper Technologies, the creator of QTY code technology, Shuguang Zhang, will share QTY applications and how Prometheus—technology for precisely measuring protein unfolding, chemical denaturation, and aggregation—may be used to look at difficult-to-characterize targets, such as amyloids and other disease-related molecules. Silvia Würtenberger of NanoTemper Technologies will share why Prometheus is the new gold standard for protein stability characterization as it tracks thermal and chemical unfolding, as well as aggregation, all in a label-free way.
Topics to be covered:
- How QTY code technology systematically replaces hydrophobic amino acids in 7 transmembrane α-helices of GPCRs
- Applying the QTY code technology to decipher the function of membrane proteins
- Examining protein stability using Prometheus
Meet the Speakers:
MIT Media Lab
Massachusetts Institute of Technology
Product Manager, Prometheus