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Technique Talk: Purifying Plant-Based Endogenous Biomolecules

Learn how reagents affect plant nucleic acids and jeopardize downstream PCR work.

The Scientist Creative Services Team
May 5, 2022

This webinar will be hosted live and available on-demand

Tuesday, June 28th, 2022
11:00 AM Eastern Time

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Nucleic acid isolation from plant tissues presents unique challenges. Commonly used solvent-based and silica-based extraction methods leave behind chaotropic salts and other inhibitors that negatively affect results in downstream applications, such as qPCR. Nucleic acid extraction from plants by negative chromatography provides better yield than traditional silica methods and reduces impurities, providing superior performance in downstream analyses.

In this Technique Talk, April Bauer will discuss negative chromatography-based purification for plant nucleic acids using GenElute™-E purification kits as well as sample preparation and PCR reagent selection for successful qPCR results.

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Meet the Instructor:

April Bauer, PhD
Product Manager
MilliporeSigma


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