ATAC-seq experiments provide scientists with genome-wide snapshots of a cell type’s epigenetic landscape at the population or single cell level. This powerful technology probes gene regulatory networks with less input material compared to other epigenetic methodologies, such as chromatin immunoprecipitation and bisulfite sequencing. However, experimental success depends on multiple factors, including sequencing depth, sample type, and sample preparation quality.
This Technique Talk will reveal how to optimize bulk and single cell ATAC-seq experiments for various sample types to obtain high quality, reproducible data.
- Preparing single cell versus bulk ATAC-seq samples
- Verifying sample quality and determining sequencing depth
- Identifying and avoiding common pitfalls
Meet the Instructor:
Claudia Lalancette, PhD
University of Michigan