Using Digital Spatial Profiling for Transcriptomic Analysis of Neuronal Subpopulations

Kit Fuhrman and Anushka Dikshit present how to obtain whole transcriptome data from spatially mapped neuron populations.

The Scientist Creative Services Team in collaboration with Advanced Cell Diagnostics

LIVE Webinar

Wednesday, December 8, 2021
2:30 - 3:30 PM Eastern Time

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Obtaining detailed molecular characterizations of neuronal populations is critical for understanding normal and pathological brain function. However, researchers working in this area are hindered by low-plex, low throughput, or an absence of spatial resolution. A combinatorial approach may be optimal for spatially-relevant whole-transcriptomic profiling.

In this webinar brought to you by ACD – a BioTechne brand and NanoString, Kit Fuhrman and Anushka Dikshit will discuss how to use RNAscope™ in situ hybridization and the GeoMx® Digital Spatial Profiler to profile excitatory and inhibitory neuronal populations within mouse brain formalin-fixed, paraffin-embedded (FFPE) sections. 

Topics to be covered

  • Performing spatial transcriptomic profiling of murine brain FFPE sections 
  • Capturing dual ISH-IF images with the GeoMx Digital Spatial Profiler to identify brain regions and neuronal subpopulations
  • How this workflow informs future profiling studies in murine neurological disease models

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Meet the Speakers:

Kit Fuhrman, PhD
Senior Product Manager
NanoString Technologies

Anushka Dikshit, PhD
Senior Scientist, Applications
Advanced Cell Diagnostics, a Bio-Techne brand

Sponsored by