Wednesday, December 8, 2021
2:30 - 3:30 PM Eastern Time
Obtaining detailed molecular characterizations of neuronal populations is critical for understanding normal and pathological brain function. However, researchers working in this area are hindered by low-plex, low throughput, or an absence of spatial resolution. A combinatorial approach may be optimal for spatially-relevant whole-transcriptomic profiling.
In this webinar brought to you by ACD – a BioTechne brand and NanoString, Kit Fuhrman and Anushka Dikshit will discuss how to use RNAscope™ in situ hybridization and the GeoMx® Digital Spatial Profiler to profile excitatory and inhibitory neuronal populations within mouse brain formalin-fixed, paraffin-embedded (FFPE) sections.
Topics to be covered
- Performing spatial transcriptomic profiling of murine brain FFPE sections
- Capturing dual ISH-IF images with the GeoMx Digital Spatial Profiler to identify brain regions and neuronal subpopulations
- How this workflow informs future profiling studies in murine neurological disease models
Meet the Speakers:
Kit Fuhrman, PhD
Senior Product Manager
Anushka Dikshit, PhD
Senior Scientist, Applications
Advanced Cell Diagnostics, a Bio-Techne brand