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fluorescence

Lipofuscin autofluorescence was quenched using EverBrite TrueBlack® Hardset Mounting Medium®, allowing effective visualization of glial cells (GFAP antibody stain, cyan) and cell nuclei (magenta) in human cerebral cortex cryosections.

Stepping Out of the Background

Illustration of newly discovered mechanism allowing kinesin to walk down a microtubule, visualized using a super-resolution fluorescence microscopy technique. A green kinesin molecule with an attached yellow fluorophore is shown passing through a blue laser as it rotates step by step along a red and purple microtubule, fueled by blue ATP molecules that are hydrolyzed into orange ADP and phosphate groups.

High-Resolution Microscope Watches Proteins Strut Their Stuff

Artist’s rendition of translucent teal proteins comprised of red spherical amino acids

Now AI Can Be Used to Design New Proteins

Fluorescence microscopy image of cells expressing fluorescent biosensors. Green and magenta fluorescence is observed outside of the cell nuclei.

Choosing Fluorescent Reagents for Every Live Cell Application

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Modernizing Drug Discovery with Flow Cytometry

To flag neurons that have experienced genotoxic stress, researchers developed an in vivo sensor using an adeno-associated viral vector, called PRISM. Because a cell’s DNA damage response (DDR)—which activates in response to stressors such as environmental toxins or the buildup of misfolded proteins—also responds to invading pathogens, PRISM has an easier time transfecting cells whose damage response mechanisms are preoccupied with existing DNA damage. Once inside, the virus hijacks the neuron’s DNA replication machinery, which reverts an engineered frameshift mutation in the virus and thereby prompts the production of a fluorescent protein that can be observed via microscopy.

Infographic: DNA Damage Viewed with Unprecedented Clarity

Broken DNA

DNA Damage Viewed with Unprecedented Clarity

Learn About the Fundamental Principles and Protocols of Flow Cytometry

Streamlining Flow Cytometry Workflows with Best Practices

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Illuminating Fluorescent Experiments

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Illuminating Biology with Luminescence

Microscopic image of nerves in the eye, a pathogen, and t cells

Science Snapshot: Eye Immunity

Trending

A drawing of men and women holding boxes, resigning en mass from their jobs as editors.

Editors Mass Resign from Elsevier Journal After Editor-in-Chief Let Go

A team of cartoon scientists studying a giant pancreas.

Prolonged GLP-1 Exposure Remodels Gene Expression in the Pancreas by Flipping a Molecular Switch

Image of a Diploptera punctata cockroach.

Cockroach Milk Proteins Churn Out an Ig Nobel Win

In the TS 40th Anniversary template, Sarkis Mazmanian smiles in a black and white headshot photo on the left. In a photo on the right, he stands in front of a large image of a cross-section of the brain. He wears a navy jacket, a white shirt, and glasses.

Then and Now: Sarkis Mazmanian Finds Gut-Brain Links to Parkinson’s Disease

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Essential Genes Are Dominantly Activated by Single Transcription Factors

Essential Genes Are Dominantly Activated by Single Transcription Factors

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Rethinking ALS Biomarkers: From Discovery to Clinical Impact

Rethinking ALS Biomarkers: From Discovery to Clinical Impact

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The Scientist Digest cover September 2026
September 2026

Multiplex Microscopy Becomes Easier with Encoded Antibodies

A new system that enables researchers to uniquely tag monoclonal antibodies for use in microscopy could help simplify complex imaging studies.

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Essential Genes Are Dominantly Activated by Single Transcription Factors

Essential Genes Are Dominantly Activated by Single Transcription Factors

EpiCypher Logo
Rethinking ALS Biomarkers: From Discovery to Clinical Impact

Rethinking ALS Biomarkers: From Discovery to Clinical Impact

Alamar Biosciences logo
Engineering CAR-Neutrophils In Vivo to Target Glioblastoma

Engineering CAR-Neutrophils In Vivo to Target Glioblastoma

Miltenyi
Best Practices for qPCR Assay Design and Optimization

Best Practices for qPCR Assay Design and Optimization

Bio-Rad

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Single cells in suspension

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Abstract image representing cell membranes linked together.

CellBrite® Steady Membrane Stain: Cell surface staining built for real-time imaging

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Monod Bio Licenses AI-designed Protein Technologies to SignalChem Biotech for Custom Discovery Assays