Cycle Sequencing Kits

When the next time around is always better. Date: July 21, 1997 Comparison Chart DNA sequencing methods have come a long way in 20 years. From the chemical method of Maxam and Gilbert and the dideoxy method of Sanger, DNA sequencing techniques evolved into the "labeling/termination" method that used a modified T7 DNA polymerase such as Sequenase. Propelled by the power and potential of DNA amplification using thermal cycling strategies, earlier DNA sequencing methodologies have increasingly b

Michael Brush
Jul 20, 1997

When the next time around is always better.

Date: July 21, 1997 Comparison Chart

DNA sequencing methods have come a long way in 20 years. From the chemical method of Maxam and Gilbert and the dideoxy method of Sanger, DNA sequencing techniques evolved into the "labeling/termination" method that used a modified T7 DNA polymerase such as Sequenase. Propelled by the power and potential of DNA amplification using thermal cycling strategies, earlier DNA sequencing methodologies have increasingly been supplanted by cycle sequencing methods.

At the heart of the thermal cycle sequencing reaction beats the eloquence of Sanger's dideoxy DNA sequencing technique. Here, a DNA polymerase synthesizes a complementary copy of a single-stranded DNA template, beginning from the 3' end of a DNA primer that has been annealed to the template strand. The Sanger dideoxy chain termination method takes advantage of the polymerase's ability to use 2',3'-dideoxynucleoside triphosphates (ddNTPs) as substrates in...

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