Gene Analyses--Sunny Side Up!

Chemiluminescent Gene Reporter Systems Features of Luminometers How is the expression of a given gene controlled? That is a question asked of many in the biological and medical sciences. "Reporter genes," which encode quantifiable enzymes or proteins, are often employed by researchers seeking to better understand how the expression of genes of interest is controlled. In these assays, the coding region of the gene under study is replaced with the sequence that encodes the reporter gene. Report

Deborah Wilkinson
Mar 28, 1999
Chemiluminescent Gene Reporter Systems
Features of Luminometers

How is the expression of a given gene controlled? That is a question asked of many in the biological and medical sciences. "Reporter genes," which encode quantifiable enzymes or proteins, are often employed by researchers seeking to better understand how the expression of genes of interest is controlled. In these assays, the coding region of the gene under study is replaced with the sequence that encodes the reporter gene. Reporter gene constructs contain one or more gene regulatory elements being analyzed; the structural sequence of the reporter gene, and sequences required for the formation of functional mRNA. Quantification of reporter gene products gives an indirect measure of the amount of gene expression obtained in a given set of conditions. Gene expression is controlled by the flanking, nontranslated "cis-acting" sequences, as well as by DNA-binding proteins and other factors that are said...

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