Histology Kits: Avidin and Biotin Team Up to Tackle the Tissue

Date: May 25, 1998 Author: Shane Beck Charts For those researchers who wish to delve into cells and tissues, there are several immunohistochemical and immunocytochemical detection methods to choose from. From the antibody-enzyme conjugation and peroxidase antiperoxidase (PAP) methods pioneered in the late 1960s came the development of avidin-biotin affinity techniques. Taking advantage of avidin's high affinity for biotin, scientists were, and still are able to look at tissues in new ways. Ear

Shane Beck
May 24, 1998

Date: May 25, 1998
Author: Shane Beck
Charts
For those researchers who wish to delve into cells and tissues, there are several immunohistochemical and immunocytochemical detection methods to choose from. From the antibody-enzyme conjugation and peroxidase antiperoxidase (PAP) methods pioneered in the late 1960s came the development of avidin-biotin affinity techniques. Taking advantage of avidin's high affinity for biotin, scientists were, and still are able to look at tissues in new ways. Early research in avidin-biotin cytochemistry by H. Heitzman and F. Richards ( Proceedings of the National Academy of Sciences U.S., 71:3537-41, 1974) and Edward A. Bayer, Meir Wilchek, and colleagues (E.A. Bayer et al., Journal of Histochemistry and Cytochemistry, 24:933-9, 1976; E.A. Bayer and M. Wilchek, Methods in Biochemical Analysis, 26:1-45, 1980) led to the development of immunohistochemical techniques still in use today.

In the 1920s, scientists discovered the protein avidin...

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