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Illuminating Images: The World of Phosphor Screen and Fluorescence Imaging Systems

Date: February 16, 1998 Chart 1 Chart 2 nyone tired of mixing darkroom chemicals should read this article. The modern generation of instruments for viewing both radioactively and fluorescently labeled samples can save you countless hours of time and provide quantitation only dreamed of by researchers in the past. The products to consider are phosphor screen and fluorescent imagers. These handy devices offer numerous advantages over film autoradiography: Sensitivity: estimates of sensitivity

Laura Defrancesco

Date: February 16, 1998 Chart 1 Chart 2
nyone tired of mixing darkroom chemicals should read this article. The modern generation of instruments for viewing both radioactively and fluorescently labeled samples can save you countless hours of time and provide quantitation only dreamed of by researchers in the past. The products to consider are phosphor screen and fluorescent imagers. These handy devices offer numerous advantages over film autoradiography:
  • Sensitivity: estimates of sensitivity are anywhere from 10 to 100 times greater than that of X-ray film.
  • Range: the dynamic range of some imagers is as great as five orders of magnitude, compared with two at best in the case of film.
  • Time: time savings come in a number of areas-preparing chemicals, exposing film, processing film, and reexposing film if the exposure is not quite right.
  • Ability to quantitate data: this is what really puts these machines in the hearts and budgets...

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