PROFILE

The Scientist 15[13]:24, Jun. 25, 2001 PROFILE Caspase Related Assays (caspases are referred to by number) Company Polyclonal Antibodies Monoclonal Antibodies Protein Substrates1 Inhibitors Assay Kit2 Accurate Chemical & Scientific Corp. (800) 645-6264 www.accuratechemical.com 3, 9, 10 Active Motif (877) 222-9543 www.activemotif.com 9 3, 7, 8, 14, pro-3 Affinity BioReagents Inc. (800) 527-4535 www.bioreagents.com 9, 10 A. G. Scientific Inc. (877) 452-9925 www.agscientific.com 1 F,C: 1–4, 6, 9 1, 2 Caspase-1 ELISA kit Alexis Corp. (800) 900-0065 www.alexis-corp.com 1–13 1–4, 6–8, 14 1–3, 6–10, Active Caspase Set F,C: 1–4, 6, 8, 9, 13 1–4, 6, 8, 9, 13, Caspase Family Sets Caspase 1 ELISA Kit AMS Biotechnology (Europe) Ltd. +44 (1235) 828-200 www.immunok.com 2–11 1, 2, 3, 8 1–3, 6–10 F,C: 1–3 , 5, 6, 8–10 1–6, 8–10, 13 Colorimetric Caspase Family Assay; F,C: 1–3, 5, 6, 8–10 Autogen Bioclear UK Ltd. +44 (1249) 819-008 www.autogen-bioclear.co.uk 1–10, 12 F: 1, 3 1, 3, 6 F: 3, 6, 8 Bachem Americas (888) 422-2436 (CA)/ (800) 634-3183 (PA) www.bachem.com F: 1, 3, 4, 6, 8, 9; C:1, 3, 6, 8; pro-3 (F,C) 1, 3, 4, 6, 8, 9, pro-3 BD Biosciences (877) 232-8995 www.bdbiosciences.com 3, 8–10 1–4, 6–10, 12, 14 3, 6–8 F: 1, 3, 6– 8 3, 6, 8, 9 Caspase-3 (F), ELISA; Caspase-6 (F), Caspase-7 (F), Caspase-8 (F); Apoptosis Antibody Sampler Kits BIOMOL Research Laboratories Inc. (800) 942-0430 www.biomol.com 1,3, 7–9 1–10, pro-3, trunc. pro-7, pro-14 Quantipak (F,C): 1–10 Casputin: specific inhibitor of 3 and 7; antisense: 3, 7 QuantiZyme Cellular Activity Assay Kit PLUS (C) for 3; QuantiZyme Assay System (C) for drug discovery of 1, 3, 8; Quantipak kits (F,C) for 1, 3, 8 Bio-Rad Laboratories - Life Science Group Div. (800) 424-6723 www.discover.bio-rad.com 1–10 1–10, two general inhibitors FluorAce Apopain Assay Kit (F) for 3 BioSource International Inc. (800) 435-2080 www.biosource.com 1, 3, 6, 8–10 8 3, 6 F,C: 1–3, 6, 8, 9 1, 3, 4, 6, 8, 9 Caspase Assay (F,C) for 2, 3, 6, 8, 9 BioVision Inc. (800) 891-9699 www.biovisionlabs.com 1–13 7, 8, 14 1–10 (active) F,C: 1–10, 13; D2R, pro-3 1–10, 13 CaspSCREEN Flow Cytometric Apoptosis Detection Kit; CaspSELECT Immunoassay Kits for 3, 7; F,C: 1–10 Boston Biologicals Inc. (888) 846-8231 www.bostonbiologicals.com 9 Calbiochem-Novabiochem Corp. (800) 854-3417 www.calbiochem.com 1, 3, 5–11, 14 3, 14 1–10, pro-3 F: 1–9; C: 1–3, 6, 8, 9, 13; general/pan substrates (F,C) 1–6, 8, 9, 13; general/pan inhibitors; caspase-3 processing inhibitor F,C:1–3,5,6,8, 10; F:9; Intracellular Activity Kit (PhiPhiLux) Cayman Chemical Co. (800) 364-9897 www.caymanchem.com 3, 9, 10 3 Cell Signaling Technology Inc. (CST) (877) 616-CELL www.cellsignal.com 3, 6–10 8 Activity Assay (F) for 7 Chemicon International Inc. (800) 437-7500 www.chemicon.com 1–3, 6–10 2, 3, 6–9 1–3, 6–10 Caspase Set of 5 Assays (F,C) for 1–6, 8–10 CLONTECH Laboratories Inc. (800) 662-2566 www.clontech.com 3 1, 3, 8 ApoAlert Assay Kits for 3 (F,C), 8 (F), 9/6 (F) DAKO Corp. (800) 235-5763 www.dakousa.com 3 Enzyme Systems Products Inc. (888) 449-2664 www.enzymesys.com 419 Anti-PARP C2.10 Anti-PARP F,C: 1, 3, 4, 6, 8, 9; 13 (F) 1–4, 6, 8, 9, 13 ApoScreen Assay (F) for 1 and 3. Exalpha Biologicals (800) 395-1137 www.exalpha.com 1, 3, 7, 9, 10, 12 3, 6–9 Geno Technology Inc. (800) 628-7730 www.genotech.com CasPASE Apoptosis Assay Kit (F,C) for 1–10 ICN Biomedicals Inc. (800) 854-0530 www.icnbiomed.com 1, 3, 7, 10; ICE-like substrate 1, 3, 7, 10; ICE-like inhibitors Imgenex (858) 642-0978 www.imgenex.com 9, 10, 12, 13 1–8, 14 Intergen Co. (800) 431-4505 www.intergenco.com CaspaTag FMK (F) for 1, 3, 6, 8, 9, general CaspaTag Caspase Activity Kits (F) for 1–3, 6, 8–10 Kamiya Biomedical Co. (206) 575-8068 www.kamiyabiomedical.com 3, 7, 9, 10 3, 6–9 3, 6–8, 10 F,C: 1–6, 8, 9 F: 1–6, 8, 9, 13; C:1, 3, 4, 6, 8 Caspase /Apoptosis Detection Assay Kit (F,C) for 1, 3 Lab Vision Corp./ NeoMarkers Inc. (800) 828-1628 www.labvision.com 3, 6–9 3 Maine Biotechnology Services Inc. (800) 925-9476 www.mainebiotechnology.com 3, 7, 9 MBL International Corp. (800) 200-5459 www.mblintl.com 6, 10 4–10 1–3, 6–10 1–6, 8–10, 13 1–6, 8–10, 13 F,C: 1–3, 5–10 Molecular Probes Inc. (800) 438-2209 www.probes.com EnzChek Assay Kits (F) for 3 Oncogene Research Products (800) 662-2616 www.apoptosis.com 1, 2, 4, 6–13, pro-1; sampler kits: CARD, general, effectors, DED caspases 3, 4, 7–9, 14 1, 3, 6–10 F:1–9; C:1,3,6,8,13; general 1, 3–6, 8, 9, 13, caspase 3-processing inhibitor, general F,C:1–3, 5, 8; F:9; C:6; HT-screening assays: 3, 8, 9; intracellular activity kits (PhiPhiLux) for 3 OncoImmunin Inc. (301) 987-7881 www.phiphilux.com F: 1, 3/7, 6, 8, 9 CaspaLux-1, CaspaLux-3 (PhiPhiLux assay system); CaspaLux-3PE (processing enzyme) PanVera Corp. (800) 791-1400 www.panvera.com 5, 7–10 1, 3, 7–9 (active) F,C: 1–3, 5–9; Caspase Family Sets 1–3, 5–10, 13; Caspase Family Sets Caspase Protease Assays (F,C) for 1–3, 5, 6, 8, 9 Promega Corp. (800) 356-9526 www.promega.com 3 (active); PARP p85 fragment Z-VAD-FMK, pan caspase inhibitor CaspACE FITC-VAD-FMK In Situ Marker; General caspase detection; Death Check I Assay System (C); CaspACE Assay System for 1 (C), 3 (F,C); Homogeneous CaspACE Assay QED Bioscience Inc. (800) 929-2114 www.qedbio.com 9, 10 R&D Systems Inc. (800) 343-7475 www.rndsystems.com 2, 3, 7, 8, 10, 11, 3 (active) 3, 8, 10 2, 3, 7, 8, 10 pan, 1–4, 6, 8–10, 13; inhibitor sampler pack Activity Assay Kits (F,C) for 1–3, 6, 8–10; Quantikine ELISA Assay for 1,3 (active) Research Diagnostics Inc. (800) 631-9384 www.researchd.com 2, 4, 6, 7, 9–11,14 1, 3, 8 Roche Molecular Biochemicals (800) 428-5433 biochem.roche.com PARP M30 CytoDEATH: binds to cytokeratin-18 Homogeneous Caspases Assay Caspase 3 Activity Assay: FIENA (Fluorometric Immunosorbent Enzyme Assay) Santa Cruz Biotechnology Inc. (800) 457-3801 www.scbt.com 1–10, 12, 14 2, 3, 8, 9 Assay Kits (F) for 3, 6, 8 SEROTEC Inc. (800) 265-7376 www.serotec.com 1, 3, 9, 10, pan-1 Sigma-Aldrich (800) 325-3010 www.sigma-aldrich.com/cellsignaling 1–3, 6–10, 13, pro-8 4, 5, 7, 10, 11 2, 3, 6–9 1–9 (F,C), 13 (F) 1–9, pro-3 Assay Kits for C:3, 6, 8; F:3, 8 STI-Signal Transduction Products (800) 701-3353 www.stisignals.com 1, 3, 7, 9, 10 Stratagene (800) 424-5444 www.stratagene.com 2 8 8 (F) 1 StressGen Biotechnologies Corp. (800) 661-4978 www.stressgen.com/reagents 2, 3, 5–10 4, 7–9 Trevigen Inc. (800) 873-8443 www.trevigen.com 3, 8, 10b, 11 (active) 2, 10a PARP 3, 8, 9 United States Biological (800) 520-3011 www.usbio.net 1, 3, 6, 8–10, 13, pro-3 8 3,9 3 (F,C), 8 (F), 9 (F) 2, 3, 6, 8, 9, pan-1, Caspase Family Inhibitor Set BioGene Kits (F,C) for 2, 3, 6, 8, 9 Upstate Biotechnology Inc. (800) 233-3991 www.upstatebiotech.com 1, 3, 6, 8–10, Caspase Variety Pack 7–9 3, 6–8, 10 F: 3, 8 C: 3, 6, 8, 9 p35 F:3 Vector Laboratories Inc. (800) 227-6666 www.vectorlabs.com 3 3, 8 Zymed Laboratories Inc. (650) 871-4494 www.zymed.com 3, 8 2, 3, 7–9 1. F=fluorogenic, C=chromogenic

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