|Date: November 10, 1997||Chart 1|
The kits profiled exploit two methods for labeling sequencing products: the incorporation of fluorescent dye-labeled primers and the incorporation of dye-labeled dideoxynucleotides (ddNTPs), or dye terminators. The use of dye-labeled primers is the most common method because the modified forms of Taq and the other novel polymerases used in these kits discriminate against dye-labeled ddNTPs and are unable to efficiently incorporate them into the reaction products. Expressly engineered polymerases, on the other hand, such as ABI's AmpliTaq DNA Polymerase, FS and Amersham's Thermo...
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