Microplate Reader Madness

Courtesy of Cellomics The ArrayScan VTI HCS Reader Microtiter plates have become standard consumables in both research and clinical laboratories. Also known as microwells and microplates, microtiter plates essentially are flat trays bearing a number of isolated reaction chambers, from six to 1,536, and arranged in a 3n x 2n array (e.g., for a 96-well plate, n=4). All the plates share a common footprint (approximately 128 x 86 mm) regardless of manufacturer and configuration, so that robot

Tariq Malik
Nov 16, 2003
Courtesy of Cellomics
 The ArrayScan VTI HCS Reader

Microtiter plates have become standard consumables in both research and clinical laboratories. Also known as microwells and microplates, microtiter plates essentially are flat trays bearing a number of isolated reaction chambers, from six to 1,536, and arranged in a 3n x 2n array (e.g., for a 96-well plate, n=4). All the plates share a common footprint (approximately 128 x 86 mm) regardless of manufacturer and configuration, so that robots, plate readers, and automated liquid handlers can easily accommodate new applications, everything from nucleic acid and protein quantification to ion-uptake analysis.

To cull data from these reactions, specialized microplate readers can use photomultiplier tubes, photodiode arrays, or charge-coupled devices (CCDs) to detect metrics such as absorption or emission, fluorescence, and luminescence. In recent years, manufacturers have even combined reading methods to build multimode instruments that increase versatility and throughput.

According to...