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GeniePrepTM Kit Ambion’s GeniePrep Kit demonstrates versatility in purifying DNA away from different buffers and contaminants including proteins, carbohydrates, RNA, oligonucleotides and DNA linkers. This one kit is designed to isolate linear and circular double-stranded DNA from a variety of sources: from E. coli, from a variety of enzyme reactions, such as restriction digestions and PCR, and DNA fragments can be isolated directly from low-melt agarose. DNA purified by GeniePrep is suitable for restriction enzyme digestion, DNA sequencing, PCR, in vitro transcription, ligation, and transformation. GeniePrep can also be used to remove unincorporated, free radioactive and nonisotopically labeled nucleotides and primers away from DNA in random-primed labeling reactions. Depending on the application, the GeniePrep procedure takes from 10 to 30 minutes. The GeniePrep Kit utilizes a glass fiber pad premounted in a spin column that preferentially binds DNA. Each of the applications follows a simple, general protocol. The DNA solution is mixed with a DNA Binding Buffer (a chaotropic salt), which is then passed through the glass fiber pad. DNA preferentially binds to the glass fiber pad while oligonucleotides, free nucleotides, and RNA pass through the fiber pad. DNA is further cleaned with three different wash solutions and then eluted with water or TE. The fiber pad has an approximately 15 microgram capacity. Nucleon Extraction and Purification Products Amersham has recently added the Nucleon DNA extraction and purification systems to their molecular biology products. These systems have a wide range of applications—plasmid purification, DNA recovery from gels, post-PCR cleanup-- and sample types, including bacteria, yeast, animal and plant tissues. Two plasmid purification kits are currently available. For minipreps, there is a spin column system –Nucleon MiP, which employs alkaline lysis followed by a bind ad elute step for rapid DNA recovery. Up to 30 micrograms of DNA suitable for sequencing and restriction digests can be recovered in 15 minutes. The large-scale kit—Nucleon MaP—utilizes a protein binding resin which does not interact with DNA and can be used with 30-500 ml cultures. PlasmidFastTM Purification Kit For plasmid purification, AMRESCO Inc. offers the PlasmidFast Purification Kit. The PlasmidFast Purification Kit is a dedicated mini-prep kit, for use with 1 ml overnight E. coli cultures. It is designed for quick purification (30 minutes) with hands-on time of only15 minutes. The kit utilizes an alkaline lysis, followed by a "selective precipitation" method for first removing specific contaminants, then concentrating purified plasmid DNA. The kit is entirely nonorganic, reagent based, and requires only a microcentrifuge for use. Each kit purifies 100 samples (1 ml each) of overnight culture and is designed to yield 2–10 micrograms of plasmid from each prep. Users have the option of using standard ethanol precipitation or a nonflammable precipitation reagent (included) for those laboratories without access to purified ethanol. The resulting plasmid DNA is suitable for use in restriction digestion, ligation, transformation, and sequencing applications. RPM® MiniPrep ExpressTM The RPM Kit provides plasmid DNA for use in any application. This kit delivers up to 20 micrograms of supercoiled plasmid DNA starting from 1.5 ml of LB culture. After alkaline lysis, plasmid DNA is selectively bound to the GLASSMILK Matrix from a cleared supernatant. Trapped contaminants are washed away and DNA is eluted in 50 microliters of water or TE. Binding, washing, and eluting take place in a microcentrifuge SPINTM Filter for maximum efficiency. With practice, twelve preps can be processed in 12 minutes. The resulting DNA is suitable for manual and automated cycle sequencing, restriction enzyme digestion, PCR, transfection, and in vitro transcription/translation. The RPM Kit comes complete with reagents and SPIN Filter/tubes for processing 60, 120, 300, or 600 minipreps. The kit is shipped and stored at ambient temperatures. For high volume applications and economy, BIO 101 also offers the MiniPrep Express Matrix to isolate plasmid DNA for restriction enzyme analysis and PCR screening purposes—at $0.08 per prep. Cleared lysate is added to the Express Matrix to bind the plasmid DNA, followed by washing and eluting. There is no need for alcohol precipitations, Rnase, or organic solvent extraction. One kit will perform 1,250 minipreps. Quantum Prep Plasmid DNA Purification Kits Quantum Prep Plasmid Purification Kits use the silicon dioxide exoskeleton of diatoms (diatomaceous earth) to bind plasmid DNA. Bio-Rad uses a special grade of this patented DNA binding matrix, and a proprietary treatment, to optimize the capture of plasmid for miniprep and midiprep purifications. The company reports that this kit maximizes the yield of high copy number plasmids from bacterial cultures and can also generate high yields of plasmid DNA from low copy number plasmids and cosmids. To insure reproducible purity, each lot of Quantum Prep kits is tested for fluorescent sequence analysis, a stringent test of plasmid DNA purity. The fluorescent sequence data generated from Quantum Prep purified plasmid DNA must be 98 percent accurate for 500 bases. Plasmid purified using the Quantum Prep matrix can be added directly to sequencing reactions without alcohol precipitations. The Quantum Prep kit uses spin filters that nest into microcentrifuge tubes. These filters allow fast and efficient washing and elution of matrix-bound DNA. The Quantum Prep protocol reduces the number of manipulations to provide a fast, simple procedure. Organic extractions and alcohol precipitations have been eliminated to speed the miniprep process. The entire procedure takes less than 15 minutes. Miniplas-MaxiplasTM Kit BioNexus Inc. has developed the Miniplas-Maxiplas Kit to provide a rapid and cost-effective system for extraction and purification of plasmid DNA from any strain of E. coli. Bacterial cells are resuspended in an enriched Resuspension solution, lysed with the Alkaline reagent, and then cellular debris and chromosomal DNA is removed by precipitating with the Acidic reagent. Plasmid DNA is subsequently precipitated from cleared lysates with room-temperature isopropanol followed by brief centrifugation. Further elimination of contaminants such as protein, RNA, and carbohydrates can be achieved by utilizing the CrystalGlas purifying matrix, which separates residual contaminants. The Miniplas-Maxiplas Kit contains 2 ml of the CrystalGlas matrix, with the binding capacity of 1 microgram nucleic acid/microliter. The company reports DNA sample recoveries of 70–90 percent. The Miniplas-Maxiplas method for extraction and purification of plasmid DNA yields a substrate that is amenable to restriction endonuclease digestion, ligation, transformation, sequencing and amplification reaction, and other molecular biology applications. The Miniplas-Maxiplas Kit can also be used for maxipreps by simply scaling up the reagent quantity used based upon the DNA quantity to be purified. BioPlazTM The BioPlaz Miniprep kit of Biotecx can isolate plasmid DNA from 1 to 3 ml E. coli cell cultures. The kit follows the standard alkaline lysis method. This system does not require organic extractions, columns, or ethanol/isopropanol precipitation. The plasmid DNA is purified by using specific DNA binding resin that binds only the plasmid DNA while RNA, proteins, and chromosomal DNA remains are washed away. Plasmid DNA is eluted from the resin with water. The recovered plasmid DNA can be used directly for sequencing and restriction digestion. High Pure Plasmid Isolation Kit The High Pure Plasmid Isolation Kit isolates small quantities of plasmid DNA from bacteria. This kit utilizes the alkaline lysis method, with the supernatant passed through a glass fiber fleece column to purify the plasmid. The resulting plasmid DNA is suitable for any subsequent experimentation, such as cloning, PCR sequencing, or random primed labeling. Twenty-four samples may be processed in forty-five minutes. All necessary solutions, except for ethanol, are supplied as well as optional wash solutions for nuclease-rich bacterial strains. Isolation of up to 10 micrograms of plasmid DNA from a 0.5 to 4 ml bacterial culture can be expected when using this kit. In addition to the Plasmid Isolation Kit, Boehringer Mannheim sells two other High Pure Kits: the High Pure PCR Purification Kit, used to clean up all enzymatic reactions, and the High Pure Template Kit, to isolate genomic DNA from bacteria, yeast, blood, cultured cells or tissues. Super CleanTM MiniPrep System SuperClean MiniPrep System is used to isolate plasmid DNA from 1 to 5 ml bacterial cultures in two steps, without using phenol or columns, and eliminates the need for column loading, washing, elution, or centrifugation. The kit comes with three buffers and enough RNase cocktail for 200 preparations. It takes only 10 minutes to prepare DNA that can be completely digested with restriction enzymes. The DNA has been tested for sequencing either using 35S-dATP or in ABI’s automatic DNA sequencer. PERFECTprep® Plasmid Isolation PERFECTprep®-96 The PERFECTprep Kit is a "single tube" approach for isolating supercoiled plasmid DNAs. The kit comes complete with tubes and processing reagents and utilizes centrifugation in combination with a silica matrix "bind-wash-elute" filtration procedure. Purified plasmid DNA is eluted from the matrix with either molecular biology grade water or TE and is ready to use without alcohol precipitation. This kit is compatible with most microcentrifuges and plasmid DNA can be purified in as little as 1.5 minutes per sample. PERFECTprep prepared plasmid DNAs are free from protein, chromosomal DNA, and RNA contaminants and can be used directly in restriction enzyme digestions, ligation with T4 DNA ligase, transformation, transfection of cultured cells, manual dideoxy DNA sequencing, automated fluorescent DNA cycle sequencing, dsDNA mutagenesis, and PCR. The high throughput PERFECTprep-96 System uses ninety-six-well format plates for the overnight growth of multiple 1-1.3 ml E. coli miniprep cultures, cleared alkaline lysate preparation, and plasmid DNA purification. Two plates (192 cultures) or four plates (384 cultures) can be processed to plasmid DNA in under 2 hours or 3 hours, respectively. Yields of supercoiled plasmid DNA will vary with plasmid copy number, host strain, and culture growing time. All plasmid DNAs prepared by PERFECTprep-96 are suitable for automated fluorescent DNA cycle sequencing (typically 600 bases or better), restriction endonuclease digestion, transfection, and PCR. JETstar JETprep Genomed's JETstar is the company’s cornerstone product for plasmid purification, while JETprep provides a quick mini-prep scale procedure. The JETstar system, which utilizes anion- exchange chromatography, is available for mini (20 micrograms), midi (100 micrograms) and maxi (500 micrograms) plasmid DNA preps. JETstar purified DNA yields sequencing data on commonly used fluorescent sequencers. With the JETstar resin, DNA can be separated from RNA without the use of Rnase. (RNase is included for optional use with each kit.) Neither special expensive equipment nor the handling of hazardous and toxic substances (e.g. phenol, chloroform or ethidium bromide) is required. JETprep uses a modified alkaline lysis procedure that forms a very compact debris pellet after the preparation of the cleared lysate, minimizing the carryover of debris particles that may inhibit subsequent enzymatic reactions. Binding of the plasmid DNA to a silica matrix in the presence of a chaotropic salt eliminates the need for an alcohol precipitation, thus making the procedure very fast. Also, large-sized plasmids (up to 15 kb) and cosmids can be purified with JETprep. A wide range (30– 90 microliters) of elution volumes is available, thereby enabling the adjustment of the final DNA concentration. Auxiliaries (syringes, vacuum chambers, etc.) are not required. JETprep eliminates the need for hazardous and toxic substances and yields plasmid DNA suitable for all standard enzymatic reactions as well as for manual sequencing. The new JetQuick Plasmid Miniprep Spin Kits are micro-spin column based. After standard alkaline lysis and centrifugation, the cleared lysate is applied onto JETQUICK spin columns, which contains a silica membrane capable of adsorbing up to 30 ug of plasmid DNA per column. Contaminants are removed by a single wash and purified plasmid DNA eluted in TE buffer or water. The procedure takes less than 20 minute and results in sequencing quality plasmid. PowerPrepTM Geno Technology has developed pinkRESINTM, a proprietary binding matrix for the purification of plasmid DNA. The company reports pinkRESIN has an enhanced binding affinity for plasmids and resuspends with ease, thus eliminating loss and damage to plasmids. Plasmid purification is based on alkaline lysis. The plasmid is allowed to bind to pinkRESIN, followed by washing steps and elution of plasmids using spin columns. The resulting plasmid is suitable for restriction enzyme analysis, fluorescent and radioactive sequencing, subcloning, transfections, and in vitro transcription. This rapid spin column protocol isolates plasmid DNA in as little as 15 minutes and yields 2–10 micrograms/prep. PowerPrep comes in two sizes, Mini Kit for 1–5 ml cultures and Large Kit for 50–100 ml cultures. For convenience, PowerPrep is supplied with all reagents, requires no vacuum manifolds or syringes, and comes with ready-to-use precast agarose Uni-LaneTM gels. S.N.A.P.TM Invitrogen offers the S.N.A.P. Miniprep Kit —a Simple Nucleic Acid Prep designed specifically for isolating plasmid DNA and optimized for automated sequencing, mammalian transfection, and other molecular biology applications. This kit eliminates phenol/chloroform extractions, long incubations, slurries, and ethanol precipitations. The entire procedure takes less than 25 minutes. The S.N.A.P. Miniprep Kit utilizes a silica-based DNA binding resin, provided in a membrane/column format. The plasmid DNA is eluted in either TE or water at 100–200 ng/microliters – the optimal concentration for automated sequencing. Coupled with the specially prepared buffers, this format allows you to efficiently bind plasmid DNA and easily centrifuge away all contaminating genomic DNA, proteins, and cellular debris. Typically, yields are greater than 10 micrograms of plasmid DNA from a 3 ml overnight culture. The procedure results in plasmid miniprep DNA that is free of contaminants such as ethanol, phenol, and salt, which can interfere with automated sequencing or transfection. ProPrepTM Plasmid ProPrep Plasmid Nucleic Acid Purification Kit is a complete nucleic acid purification system based upon a unique protein-binding reagent, ProCipitateTM. DNA is not bound, providing high yields in the purification of plasmid DNA. The ProPrep system utilizes a modified alkaline lysis procedure with a RNAse cocktail to yield DNA suitable for automated fluorescent sequencing, Southern blotting, and enzyme restriction. The kit comes in two sizes, 100 and 1,000 sample sizes. In conjunction with ProPrep Plasmid, Ligochem can supply, at the customer’s option, high throughput 96 well filter plate system designed specifically for use with the ProPrep Plasmid system. This high throughput format lyses, neurtralizes, precipitates and filters samples within the same well. UltraCleanTM Mini Plasmid Prep MoBio Laboratories has developed the Mini Plasmid Prep Kit, which yields automated sequence quality plasmid DNA in 6 minutes. The UltraClean Mini Plasmid Prep Kit is based on alkaline lysis with a specially developed Neutralizer that pellets the SDS precipitate into a tight pellet that doesn’t need to be filtered. Following that, the plasmid DNA is bound to a silica membrane spin- filter, washed once, and eluted as pure plasmid DNA. No silica matrix is used. All reagents are completely prepared so no preliminary preparation steps are required. Even the ethanol in the wash is already prepared. This kit provides all necessary tubes so searching for tubes that match up with the spin filter is not necessary. DNA quality and purity are equal to those resulting from cesium chloride or anion exchange methods. The company has determined that the yield is 100 percent of the plasmid contained in a 2 ml culture. The kit is compatible with high nutrient growth media, which can increase yields by up to seven times the yield in LB media. UltraClean DNA can be used for automated sequencing, transfections, restriction digest, labeling, PCR, ligations. MoBio Laboratories will provide free samples of its kit for comparison with the method you currently use. MaxiPlas/GlasPac QuicKit The MaxiPlas/GlasPak QuicKit from National Scientific employs the alkaline lysis purification method to remove cellular debris and chromosomal DNA. Protein and oligo RNA are removed in a glass binding step utilizing National Scientific’s proprietary high binding glass to obtain purified plasmid DNA suitable for sequencing and cloning procedures. The MaxiPlas/GlasPac QuicKit was designed for versatility and economy. The MaxiPlas/GlasPac QuicKit can isolate a variety of DNA’s without having to resort to multiple specialized kits. Pure plasmid DNA, genomic DNA, and PCR fragments may be isolated from solutions or gels at a cost of 20 to 80 cents per sample. The protocol is easily modified to maximize parameters such as speed, quality, quantity, and concentration to permit the most efficient means to be employed for a particular goal. BioMag® Mini-Prep DNA Purification Kits The BioMag Mini-Prep DNA Purification Kits from PerSeptive Biosystems enable rapid and cost-effective purification of sequencing-grade plasmid DNA from bacterial cultures. The kits use BioMag carboxyl terminated magnetic particles to nonspecifically bind DNA under conditions of high polyethylene glycol and salt (SPRI, solid phase reversible immobilization technology). This method works well for the purification of all types of DNA from plasmids to single copy BAC clones. Overnight bacterial cultures are lysed using a standard protocol, and BioMag particles are added to the supernatant containing plasmid DNA. The DNA then binds to the BioMag particles and is washed by buffer exchanges using rare earth magnets supplied by PerSeptive Biosystems. The purified DNA is eluted from the BioMag particles with Tris buffer. BioMag Mini-Preps do not require any vacuum apparatus or toxic phenol/chloroform, are scaleable to both small and larger volume cultures, and isolate DNA ready for direct sequencing. The SPRI technique allows the manipulation and isolation of all types of double-stranded DNA using a single protocol and is amenable and compatible with automation. The BioMag Mini-Prep DNA Purification Kit is available in a ninety-six well format and in a microcentrifuge tube format. The yield of DNA for both kits is approximately 50 ng/microliters for multi-copy double-stranded plasmid DNA and 30 ng/microliters for cosmid DNA. GFXTM Micro Plasmid Prep Kit GFX Micro Plasmid Prep Kit, from Pharmacia Biotech, is designed for the isolation of plasmid DNA from multiple samples. Based on a modified alkaline lysis method and selective binding to a glass fiber matrix, the GFX Kit yields up to 6 micrograms of plasmid DNA per ml of culture from 1.5 ml of overnight culture. Plasmid DNA purified using GFX Micro Plasmid Prep Kit can be used directly in manual and automated* sequencing, PCR, mutagenesis, library screening, subcloning, transformation, labeling and restriction analysis. The MicroSpinTM column format can be used to process multiple samples using MicroPlex® 24** Vacuum or single samples using a standard microcentrifuge. When used with MicroPlex 24 Vacuum, forty-eight samples can be processed in about an hour. The columns can be used with 1–3 ml culture volumes. GFX columns are ready to use, and the GFX purification method avoids using slurries, syringes, organic reagents, and ethanol precipitations. GFX Micro Plasmid Prep Kit is available in two pack sizes, sufficient for processing either 100 or 250 standard minipreps from 1.5 ml of culture. FlexiPrep Kit is available for the purification of plasmid DNA from larger volumes up to 500 ml. FlexiPrep Kit uses a scalable, glass-based purification method that can accommodate culture volumes from 1.5 ml to 500 ml. *For automated sequencing, DNA may need to be precipitated to achieve the required concentration. **U.S. Patent No. 5,603,899 has been issued to Pharmacia Biotech Inc. for multiple column chromatography assembly. PSI CloneTM Plasmid Prep Kit Princeton Separations offers the PSI Clone Plasmid Prep Kit, providing plasmid isolation and rapid buffer exchange in one kit. The company focus has been on eliminating steps that can lead to loss of plasmid. When using a nonsilica resin that reportedly allows for 80–90 percent elution of plasmid, yields of up to 25 micrograms can be obtained. Incorporated into the kit are CENTRI-SPIN columns, which accomplishes rapid desalting/buffer exchange. Plasmid concentrations as high as 500 ng/microliter can be obtained without ethanol precipitation or membrane concentration. Purified plasmid is compatible with common next steps, such as sequencing, subcloning, PCR and transfections. Princeton Separations also offers the CENTRI-SEP spin column, a widely used column in dye-terminator DNA sequencing. Super Mini-Easy Pure Plasmid Prep The Super Mini-Easy Pure Plasmid Prep provides a simple method for the rapid isolation of plasmid DNA from a 3 ml starting volume of bacterial culture. Using a DNA binding resin, plasmid DNA is purified and recovered. The resin is then collected in a special spin filter basket and the DNA eluted with water or TE buffer. The purified DNA is free of proteins, RNA, salts or other contaminants and can be used directly for automated sequencing, cloning and other molecular biology procedures, without further precipitation or phenol extraction. Features of the Super Mini kit include the ability to scale up with yields up to 50 micrograms of pure DNA; sample preparation that allows for the processing of 24– 48 samples using a microcentrifuge; no requirement for syringes or vacuum manifolds; the testing of all kit reagents for their reproducibility during automated sequencing using the ABI Automated Sequencers; and the recovery of Form 1 DNA. Other DNA purification products include Super Midi prep kit which allows for the rapid purification of twenty four samples in one hour; the Super Maxi prep kit, used when 1–1.5 mg of DNA is required; and the DNA extraction kit, used for rapid extraction of DNA from agarose gel slices. Wizard® Plus SV Miniprep DNA Purification System The Wizard Plus SV Miniprep DNA Purification System from Promega allows a choice of plasmid purification formats: "Spin" or "Vacuum" ("SV"). Plasmid DNA may be purified from the bacterial lysate through the Wizard Plus Miniprep Spin Column and then washed and eluted. Alternatively, the bacterial lysate can be pulled through the Wizard Plus SV Miniprep Spin Columns using a vacuum format. The vacuum adapters allow the use of the VacMan® Laboratory Vacuum Manifold to purify plasmid DNA. Purified plasmid can be used directly for automated fluorescent DNA sequencing or restriction digestion without further manipulation and also can be used for in vitro coupled transcription/translation reactions such as TNT® T7 Quick System (cat. # L1170). Each lot is tested to ensure that Wizard Plus purified DNA analyzed with an ABI Model 377 DNA Sequencer meets or exceeds the target accuracy over 500 bases in fluorescent sequencing. Qiagen's BioRobot 9600: automates proven technologies. QIAGEN® Plasmid Miniprep Kit QIAwell® 8, 8 Plus, 8 Ultra Plasmid Miniprep Kits QIAprep® Spin, 8 Plasmid, 8 Turbo, 96 Turbo Miniprep Kits QIAwell® 96 Ultra Plasmid Miniprep Kit R.E.A.L.TM Prep 96 Plasmid Miniprep Kit QIAGEN has developed a range of miniprep kits to meet different DNA quality, throughput and cost requirements. Purification procedures incorporating QIAGEN Anion-Exchange Resin provide their highest level of DNA purity. DNA binds tightly to the resin by charge interaction, allowing complete removal of contaminants. For smaller sample numbers, QIAGEN Plasmid Miniprep Kits use gravity flow columns to purify DNA. For larger sample numbers, the QIAwell Plasmid Purification System uses QIAGEN Anion-Exchange Resin in a membrane form for rapid sample processing on the QIAvac Vacuum Manifold. The QIAwell System includes accessory modules for rapid lysate clearing and sample desalting and concentration. QIAwell Plasmid Kits are available in eight well and ninety- six well formats. DNA purified with the QIAGEN Plasmid Mini or QIAwell Plasmid Kits is recommended for all applications. QIAprep Plasmid Miniprep Kits use silica gel-based membranes to produce low-cost plasmid DNA. Plasmid DNA bound to a silica membrane is eluted in a low ionic strength buffer, ready for use. QIAprep Spin Minipreps provide flexible handling options—minipreps can be performed with either a centrifuge or vacuum manifold. For larger sample numbers, QIAprep 8, QIAprep 8 Turbo, and QIAprep 96 Turbo Miniprep Kits provide high throughput sample processing in 8- or 96 well formats. QIAprep Turbo Miniprep Kits feature TurboFilterTM modules for rapid lysate clearing. For very high throughput sample processing, R.E.A.L. Prep 96 Kits can be used to purify up to 4 x 96 samples in less than 90 minutes. Cultures are grown and lysed in a ninety-six well block, cleared by vacuum filtration, and concentrated by isopropanol precipitation. Plasmid DNA purified with this kit is suitable for fluorescent automated sequencing under high-throughput production conditions. Elu-Quik® DNA Purification Kit The Elu-Quik DNA Purification Kit from Schleicher & Schuell, Inc. purifies plasmid DNA using an alkaline or boiling mini-prep procedure followed by binding DNA to glass particles in the presence of sodium perchlorate binding buffer. The kit utilizes uniform glass rods that minimize shearing and are claimed to be gentler on the DNA than glass used in other systems. The optimized buffer system may provide higher yields since there is no irreversible binding of DNA as can occur with sodium iodide. A wash buffer is used to eliminate contaminants, followed by a salt reduction buffer that increases yields by 25 percent and reduces salt by 100 times in the final sample. Elution of the DNA from the glass particles is in TE or water; no ethanol precipitation is necessary. The eluted material is ready for restriction enzyme digestions or other procedures, and contaminating transfer RNA is eliminated. The Elu-Quik kit contains glass concentrate, binding buffer, wash buffer, salt reduction buffer, nonshear pipette tips, and floating tube rack. Sufficient reagents are included for 250 mini-prep isolations per kit. PlasmidPURETM DNA Miniprep Kit The PlasmidPURE DNA Mini-prep Kit is a complete plasmid purification system for isolation of plasmid DNA. The kit utilizes PlasmidPURE spin filters and an optimized buffer system to isolate as much as 15 micrograms of purified plasmid DNA from 1–3 ml of an overnight bacterial culture. The PlasmidPURE kit does not require the use of phenol, chloroform, cesium chloride, syringes, or vacuum manifolds. Based on a modified alkaline lysis procedure, the purification scheme is performed in a few steps. After lysis, plasmid DNA is isolated from chromosomal DNA and cell debris, bound to the PlamidPURE spin filter, and washed to remove impurities. Finally, plasmid DNA is eluted into either a modified TE solution, included in the kit, or in water. The result is plasmid DNA that is ready for immediate use in restriction and modifying enzyme protocols and in sequencing reactions. The PlasmidPURE miniprep kit performs well on both high and low copy number plasmid, yielding predominantly supercoiled DNA. ClearCutTM Miniprep Kit The ClearCut Miniprep Kit provides a reliable protocol for preparing plasmid miniprep DNA using a modification of the alkaline-lysis method. The total yield of pBluescript® phagemid DNA in XLI-Blue cells ranges from 5 to 10 microgram from a 1.5 ml overnight culture. The ClearCut kit uses a column of silica resin to rid the DNA of contaminants. The kit has multiple applications; it can be used to remove contaminants resulting from multiple enzymatic manipulations or to remove impurities or conflicting buffers from the previous experiment. After the 20-minute ClearCut purification procedure, the DNA is ready for restriction, ligation, and sequencing reactions. The kit’s solution drip bottles provide sample-volume consistency. Furthermore, because pipets are not dipped into drip bottles, the chance of solution contamination is significantly reduced. The ClearCut Miniprep Kit comes with ClearCut solutions in drip bottles, DNA binding resin, wash buffer, and spin cups with receptacle tubes. Plasmid MiniPrep Kit USBiological offers a number of kits for purifying plasmid DNA with a choice of purifying agent, either anion exchange column chromatography (gravity) or cation exchange spin columns, as well as a choice of culture size -- from 1 to 25 ml. The Plasmid Mini Prep Kit with Cation Exchange Spin columns contains all the solutions, buffers and Mini Prep spin columns with capacity to purify 1 ml to 25 ml of culture each. This kit is suited for larger cultures due to the design which allows for purification of 5 ml to 25 ml cultures in the same time it ordinarily takes to purify 1 ml. Following conventional alkaline lysis, the cleared supernatant is subjected to cation exchange chromatography to remove contaminating proteins resulting from the alkaline lysis procedure. The high mass ratio between the ion exchange and the contaminating proteins makes possible the use of spin column chromatography which is faster and easier to perform that gravity driven flow column chromatography or vacuum manifold methods. While this kit works with as little as 1 ml of culture, the manufacturers recommend a minimum of 5 ml culture. Excluding time necessary for culture growth, the whole purification process can be completed in less than one hour for cultures of 1 to 25 ml Comparison Chart Laura DeFrancesco can be reached at ldefrancesco@the-scientist.com

Mini Preps Are Here To Stay So Say The 25 Companies Who Make Kits.

Aug 17, 1997

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