Mutants Made Easy

Suppliers of in vitro Site-directed Mutagenesis Kits Biological research greatly benefits from the ability to introduce specific mutations into a DNA sequence. Researchers use site-directed mutagenesis procedures to precisely analyze individual amino acid residues in a protein sequence and in specific protein-nucleic acid interactions. Likewise, serial deletion and random insertion protocols can ease protein structural studies and promoter analyses. In their original incarnations, site-directe

Jeffrey Perkel
Feb 4, 2001

Suppliers of in vitro Site-directed Mutagenesis Kits

Biological research greatly benefits from the ability to introduce specific mutations into a DNA sequence. Researchers use site-directed mutagenesis procedures to precisely analyze individual amino acid residues in a protein sequence and in specific protein-nucleic acid interactions. Likewise, serial deletion and random insertion protocols can ease protein structural studies and promoter analyses.

In their original incarnations, site-directed mutagenesis protocols required the isolation of single-stranded DNA (ssDNA) from bacteriophage (bacterial viruses), followed by annealing of a primer that was complementary to the target DNA except at the site to be mutated, and the synthesis of a complete "mutant" second strand by a DNA polymerase. Finally, the resultant heteroduplex plasmids were ligated and transformed into bacteria. With no additional enrichments, this protocol theoretically yields a 50 percent mutagenesis frequency; in practice, however, the rate is much lower and results in the need to screen many...