Courtesy of Applied Biosystems |
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In the nearly two decades since its discovery, the polymerase chain reaction (PCR) and its derivative techniques have revolutionized molecular biology. The exponential nature of the technique allows one, in theory, to calculate the amount of starting material from the amount of product at any point in the reaction. In practice, however, reaction conditions can interfere with the exponential amplification and affect product concentration. Standard PCR works best, therefore, as a qualitative...
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