Most researchers would agree that manually performing a hundred or so plasmid preps after a low-efficiency cloning stifles the spirit of exploration that attracted them to science. Minipreps don't end with merely being tedious, repetitive, and time consuming--frequent exposure to hazardous chemicals adds to the misery. However, minipreps are a necessary evil in research, and the number of sequencing templates to prepare never seems to dwindle. For example, a small lab running four sequencing gels a day with a 36-well comb can accommodate 144 samples a day; a larger operation running just one 96-capillary machine around the clock can analyze 1,000 templates daily. Preparing templates to meet this demand is challenging.
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