Tissue Microarrays: Advancing Clinical Genomics

Image: Courtesy of Biocat SCORES OF CORES: Each tissue core on this microarray provides another datapoint that helps researchers better define the molecular characteristics of interesting genes. In 1997, Juha Kononen, a postdoctoral fellow at the National Human Genome Research Institute, was pondering the significance of the recently developed DNA microarray. He was studying genetically altered genes in cancerous cells using fluorescence in situ hybridization and immunostaining of indivi

Jeffrey Perkel
Oct 27, 2002
Image: Courtesy of Biocat
 SCORES OF CORES: Each tissue core on this microarray provides another datapoint that helps researchers better define the molecular characteristics of interesting genes.

In 1997, Juha Kononen, a postdoctoral fellow at the National Human Genome Research Institute, was pondering the significance of the recently developed DNA microarray. He was studying genetically altered genes in cancerous cells using fluorescence in situ hybridization and immunostaining of individual tissue sections. The process was, he says, "very laborious." Kononen wondered whether a technology based on DNA biochips could aid in his research. "I thought why could you not invert the concept? Instead of laying down hundreds or thousands of probes, how about laying down hundreds or thousands of tissue spots and probing them one antibody or gene probe at a time." Kononen ap-proached his advisor, Olli Kallioniemi, NHGRI section head for the cancer genetics branch, who gave the green...