Tyrosine Phosphorylantion State-Sensitive Antibodies Protein tyrosine kinases (PTKs) are a diverse lot, including both transmembrane and soluble cytoplasmic enzymes. Roughly 10 percent of a cell's proteins are subject to covalent modification via phosphorylation, but tyrosine (Tyr) residues are modified in only about one out of every 100 protein phosphorylation events. When Tyr-phosphorylation occurs, cells pay heed. PTKs play vital roles in a wide variety of cellular processes, including growth, differentiation, and metabolism.
If you are in the business of detecting, visualizing, or purifying Tyr-phosphorylated proteins, then antiphosphotyrosine antibodies [ anti-(P)Tyrs] will definitely be on your shopping list. Several types of anti-(P)Tyrs are commercially available. Polyclonal preparations are sold by various vendors, and a number of different monoclonal anti (P)Tyrs are also on the market. Most of the currently available anti-(P)Tyrs bind free (P)Tyr quite well--with affinities in the µM range...
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