When You Wish Upon A Star: Molecular Beacons: Real Time in a Twinkle

Table of Licensed Providers of Molecular Beacons and Kits Using molecular beacons for spectral genotyping. Differently-colored molecular probes specific for the wild-type and mutant alleles are designed. DNA amplified from homozygous wild-type individuals binds only to the fluorescein-labeled molecular beacons (left). DNA from homozygous mutants binds only the tetramethylrhodamine-labeled molecular beacons (right). Both types of molecular probes will bind to amplicons generated from the DNA

Deborah Wilkinson
Mar 1, 1999
Table of Licensed Providers of Molecular Beacons and Kits


Using molecular beacons for spectral genotyping. Differently-colored molecular probes specific for the wild-type and mutant alleles are designed. DNA amplified from homozygous wild-type individuals binds only to the fluorescein-labeled molecular beacons (left). DNA from homozygous mutants binds only the tetramethylrhodamine-labeled molecular beacons (right). Both types of molecular probes will bind to amplicons generated from the DNA of heterozygous individuals (center). Reprinted with permission from L.G. Kostrikis et al., "Spectral genotyping of human alleles," Science 279,1228-9, 1998. Copyright 1998, American Association for the Advancement of Science
The annealing of a nucleic acid strand to its complement is one of the most specific molecular recognition events known. A plethora of approaches exploiting this fact have been used for basic research. For example, nucleic acid blotting techniques have been used to make great strides in our understanding of gene organization and function. In...

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