What is it? Acrydite is a molecular chimera containing an acrylic group and phosphoramidite that can be used as a platform for oligo construction (via the phosphoramidite part) and as a vehicle to attach oligos to surfaces (via the acrylic portion). What makes this particular molecule so useful is that it attaches to surfaces in a single step--and a number of different surfaces are possible--forms a bond that is resistant to high temperatures, and leaves the attached oligo accessible for hybridization assays that are both rapid and efficient.
Five layer gel with horizontal capture layers A-E. The five samples of fluorescently labeled oligonucleotides are each complementary to only one of the five capture probes. At left, a mixture of all five are loaded in the same well.
Interested in reading more?
Become a Member of
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member?