Cytokines, unique growth factors that are secreted by various cells of the body, include interleukins (ILs), interferons (IFNs), and tumor necrosis factors (TNFs). These proteins bind to target cell surface receptors and activate cell proliferation and/or differentiation. Until recently, research that quantifies levels of cytokine-specific mRNA using traditional methods such as the northern blot, slot blot, quantitative RT-PCR, and ribonuclease protection assay (RPA) has been labor-intensive and time-consuming. Additionally, these methods are either unsuitable for high-throughput analyses or require the use of a radioisotope.
R&D Systems' Quantikine mRNA kit
Interested in reading more?
Become a Member of
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member?