Protein-protein interaction is a key concept in the fields of signal transduction and gene regulation. Investigators can make educated guesses as to whether two proteins actually interact, but verifying these interactions is not a trivial task. Classical biochemical techniques are fraught with pitfalls that make data acquisition and interpretation difficult. BioSignal Packard, a division of Packard Instruments, has introduced the BRET2 (Bioluminescence Resonance Energy Transfer) system to assess these protein-protein interactions in vivo.
Components of the BRET2 system