Through the years, a variety of separation techniques have been used by researchers in their continuing challenge to accurately separate and identify analytes of interest from complex mixtures. Numerous procedures, physical as well as chemical, conducted in an assortment of vessels, are often used to achieve this goal. During the past 25 years, column techniques such as gas chromatography (GC) and high-performance liquid chromatography (HPLC) have led to successful separation and identification of thousands of compounds.
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