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Card Tricks! DAKO's GenPoint(TM) Can Detect Single Copy Sequences Without PCR or Radioactivity

Visualization by DAKO GenPointTM of in situ hybridization of a biotinylated probe to the cyclin-D homolog gene of Kaposi's Sarcoma Herpes Virus/Human Herpes Virus 8 to a cutaneous lesion of Kaposi's sarcoma. Photograph courtesy of Dr. Jon A. Reed, M.D., Department of Pathology, The New York Hospital-Cornell Medical Center, N.Y. If you've ever struggled with in situ PCR, read on. DAKO has introduced a new product for amplifying in situ hybridization signals that uses neither radioactivity nor P

The Scientist Staff


Visualization by DAKO GenPointTM of in situ hybridization of a biotinylated probe to the cyclin-D homolog gene of Kaposi's Sarcoma Herpes Virus/Human Herpes Virus 8 to a cutaneous lesion of Kaposi's sarcoma. Photograph courtesy of Dr. Jon A. Reed, M.D., Department of Pathology, The New York Hospital-Cornell Medical Center, N.Y.
If you've ever struggled with in situ PCR, read on. DAKO has introduced a new product for amplifying in situ hybridization signals that uses neither radioactivity nor PCR. This novel technology called catalyzed reporter deposition, or CARD, has been incorporated into the GenPoint product for amplifying signals from rare sequences.

Catalyzed Signal Amplification, or CSA as it is called by DAKO, is a method for adding successive layers of biotin to a sample hybridized with biotinylated probes through a catalytic process, rather than the more common sandwiching of layers of biotin/avidin molecules. By using catalysis, the potential for amplification...

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