Companies produce DNA sequencing kits with the expectation that their kits will outperform their competitors' kits. While the majority of available kits boast better performance and superior results and have pictures to prove it, it is not always possible to reproduce the promised results of some kits. It has been my experience that you can't tell how good a kit is until you try the protocol yourself and see if the outcome is as advertised. Most importantly, though, does the kit you selected perform the DNA sequencing that you set out to do? I have found one kit that not only meets all of its advertised expectations but can be modified to suit my own special nonstandard sequencing needs as well.
|Comparison of DNA sequencing reactions conducted with standard kit (left) showing a comparison in the boxed region, and with SequiTherm EXCELTM II from Epicentre (right), showing resolution of...|