The ability of cells to take up foreign DNA is one of the most relied-upon properties in molecular biology. Electroporation uses electrical field pulses to create pores in the cell membrane through which foreign material can be introduced. One of the newest and most innovative instruments for electroporation is the Cloning Gun from Tritech Research.
Tritech's Cloning Gun
The Cloning Gun is a cordless, rechargeable, handheld device weighing less than 1 lb. The user simply pipettes cells into the unique, disposable Pipectrode™
, pulls the trigger, and ejects transformed/transfected cells into the appropriate vessel. The MammoZapper™
version transfects a variety of commonly used mammalian cell lines with an efficiency of greater than 50 percent of viable cells. The BactoZapper™ version can be used to obtain up to 10 billion Escherichia coli
transformants per microgram of input DNA.
Vinodh Narayanan, principal investigator in the department of neurology at Children's...