Fluorescence Spectroscopy Methods Reveal Biomolecules' Dynamics

Dynamics Author: Howard Goldner During the past few decades, fluorescence spectroscopy has developed into an integral technique in many scientific disciplines. In the life sciences, it is implemented extensively in areas such as biochemistry, biophysics, and cell biology for a variety of applications, ranging from basic assay-related quantitative measurements to DNA sequencing. Locates Molecules:Fluorolog-t2 Spectrometer More recently, advances in instrumentation, laser technology, and fluore

Howard Goldner
Jun 11, 1995

Dynamics Author: Howard Goldner During the past few decades, fluorescence spectroscopy has developed into an integral technique in many scientific disciplines. In the life sciences, it is implemented extensively in areas such as biochemistry, biophysics, and cell biology for a variety of applications, ranging from basic assay-related quantitative measurements to DNA sequencing.


Locates Molecules:Fluorolog-t2 Spectrometer

More recently, advances in instrumentation, laser technology, and fluorescent dyes (or probes) are aiding researchers in their efforts to better understand the dynamics of complex biomaterials such as proteins, membranes, and nucleic acids. Fluorescence- microscopy methods, for example, are increasingly being used to study the localization and movement of intracellular substances (H. Ahern, The Scientist, April 17, 1995, page 18).

"Scientists like to use fluorescence spectroscopy for two reasons," explains William Mantulin, an adjunct associate professor of biochemistry and biophysics and the director of the Laboratory for Fluorescence Dynamics, a National Institutes of...

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