There is a war going on to win over the hearts and minds of molecular biologists: Radioactive isotopes-long the gold standard for tagging and later detecting RNA and DNA strands- are being challenged by a new generation of fluorescent labels that promise greater flexibility with fewer disposal problems.
SEQUENCE DETECTION SYSTEM: Perkin-Elmer’s ABI Prism 7700 system uses a probe with a reporter and a quencher dye attached to it.
That endearing quality helped boost radiolabels to the forefront of molecular biology techniques. Labeled oligonucleotides will pair with complementary stretches according to the Watson-Crick model: Guanine on one chain links to a...
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