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Flyin' Ion Exchange

Process of proteins flowing through channels of Continuous Bed. Loading-whether it's a computer program, the station wagon in preparation for a big vacation, or an ion exchange purification, nobody likes to wait. "The faster you can load the column without losing any resolution, the better off you're going to be. In all I've done, [sample loading] has always been the limiting step," says Anthony David Couvillon, lab manager in the division of signal transduction at Beth Israel Deaconess Medic

James Kling


Process of proteins flowing through channels of Continuous Bed.
Loading-whether it's a computer program, the station wagon in preparation for a big vacation, or an ion exchange purification, nobody likes to wait.

"The faster you can load the column without losing any resolution, the better off you're going to be. In all I've done, [sample loading] has always been the limiting step," says Anthony David Couvillon, lab manager in the division of signal transduction at Beth Israel Deaconess Medical Center in Boston, Massachusetts. Loading a column often takes longer than the actual run.

With that in mind, Bio-Rad has introduced its new UNO ion exchange columns for biomolecule purification. They're packed with a novel "Continuous Bed" matrix that promises greatly increased flow rates-and that makes sample loading a lot less painful says Couvillon. He started loading the column at his typical flow rate of 1/2 ml per minute, slowly raised...

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