Getting Radical

HeLa lysates were oxidized by the addition of H2O2 (hydrogen peroxide) for 0, 0.5 or 3.5 hours. Afterwards, cell lysates from the control or H2O2-treated samples were treated +/- dinitriphenylhydrazine, then separated by PAGE and transferred to a nitrocellulose membrane. Dinitrophenylated proteins were immunodetected using the anti-DNP antibody provided in the kit. The lanes contain: (1) dinitrophenylated molecular weight standards; (2) DNP-modified HeLa control cells; (3) DNP-modified, 0.5-hou

Deborah Wilkinson
Feb 1, 1999

HeLa lysates were oxidized by the addition of H2O2 (hydrogen peroxide) for 0, 0.5 or 3.5 hours. Afterwards, cell lysates from the control or H2O2-treated samples were treated +/- dinitriphenylhydrazine, then separated by PAGE and transferred to a nitrocellulose membrane. Dinitrophenylated proteins were immunodetected using the anti-DNP antibody provided in the kit. The lanes contain: (1) dinitrophenylated molecular weight standards; (2) DNP-modified HeLa control cells; (3) DNP-modified, 0.5-hour H2O2-treated HeLa cells; (4) DNP- modified, 3.5 hour-H2O2-treated HeLa cells; (5) unmodified HeLa control cells; (6) unmodified HeLa, 0.5-hour H2O2-treatedHeLa cells. Reprinted with permission from Intergen.


Free radicals have been implicated in the oxidative modification of a wide variety of macromolecules, including nucleic acids, lipids, carbohydrates, and proteins. "During the last 5 to 6 years, increasing attention has been paid to the oxidation...

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