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Stratagene's Vitality™ hrGFP (green fluorescent protein) mammalian expression vectors allow protein expression and subcellular localization studies similar to those using jellyfish (Aequorea victoria) GFP, with one important difference: Stratagene's hrGFP exhibits less toxicity in many mammalian cells. Renilla reniformis, an Anthazoan sea pansy related to the jellyfish, produces a 238 amino-acid protein that has broader pH stability, more resistance to solvents, detergents, and proteases,

Bob Sinclair

Stratagene's Vitality hrGFP (green fluorescent protein) mammalian expression vectors allow protein expression and subcellular localization studies similar to those using jellyfish (Aequorea victoria) GFP, with one important difference: Stratagene's hrGFP exhibits less toxicity in many mammalian cells.

Renilla reniformis, an Anthazoan sea pansy related to the jellyfish, produces a 238 amino-acid protein that has broader pH stability, more resistance to solvents, detergents, and proteases, and a 2.5-fold higher extinction coefficient than does the red-shifted Aequorea protein. While it has less than 30 percent homology to the Aequorea protein, the hrGFP chromophore is similarly derived from a post-translational cyclization of a tripeptide and also exhibits an emission maximum at just over 500 nm. For expression studies and vector construction, La Jolla-based Stratagene constructed a synthetic gene that uses codons preferred in highly-expressed human genes.


Stratagene's Vitality hrGFP vectors
Toxicity from Aequorea GFP leads to problems in many...

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