Going for Gold

Traditional array detection systems employ fluorescence or radionuclide labeling, but these methods can lead to photobleaching or high background noise. Valencia, Calif.-based QIAGEN recently launched the HiLight™ Array Detection System to address this problem. The HiLight system uses a method called Resonance Light Scattering (RLS), which takes advantage of the light-scattering properties of nanoscopic metal particles in suspension. When irradiated with white light, these particles sca

Aileen Constans
Oct 27, 2002

Traditional array detection systems employ fluorescence or radionuclide labeling, but these methods can lead to photobleaching or high background noise. Valencia, Calif.-based QIAGEN recently launched the HiLight™ Array Detection System to address this problem.

The HiLight system uses a method called Resonance Light Scattering (RLS), which takes advantage of the light-scattering properties of nanoscopic metal particles in suspension. When irradiated with white light, these particles scatter light at different wavelengths, depending on particle size, shape, and composition. A solution of 40-nm gold nanoparticles, for example, scatters green light in a manner similar to a fluorescing fluorescein solution and can thus be used as a fluorescence analog in applications in which fluorescent detection is traditionally used.1

Because the HiLight system uses metal particles rather than chemical labels, photobleaching is eliminated, leading to higher signal-to-noise ratios. This dramatically increases the sensitivity of the system over current array detection methods, explains marketing...

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