Procedure for Cyclex's Luminetics T cell activation assay
In the Luminetics Assay, T cells are stimulated with an antigen or mitogen first, followed by separation of specific T-cell subsets. Monoclonal antibodies attached to paramagnetic particles recognize specific cell surface markers, and T cells are isolated when the sample is placed in a magnetic field. Because increased intracellular ATP accumulation is associated with T-cell activation, a luminescent ATP assay employing firefly luciferase measures the level of activation among isolated cells. As Peter Sottong, vice president and director of operations at Cylex, explains, "By comparing the activated to the unstimulated cells, you can get a sense of the health of the immune system itself."
Researchers can use the Luminetics Assay for a variety of applications, including AIDS, autoimmune disease, cancer, and organ transplantation research. In addition, simply changing the monoclonal antibodies attached to the magnetic beads enables study of other lymphocyte subsets.
--Aileen Constans (email@example.com)
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